食品科学 ›› 2010, Vol. 31 ›› Issue (23): 188-193.doi: 10.7506/spkx1002-6630-201023043

• 生物工程 • 上一篇    下一篇

酪蛋白水解物对瑞士乳杆菌生长的影响

胡志和1,区翠颜1,朱利民2,冯永强2,张建军2   

  1. 1.天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院 2.天津海河乳业有限公司
  • 收稿日期:2010-09-14 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 胡志和 E-mail:hzhihe@tjcu.edu.cn

Effect of Casein Hydrolysate on Promoting the Growth of Lactobacillus helvecticus

HU Zhi-he1,OU Cui-yan1,ZHU Li-min2,FENG Yong-qiang2,ZHANG Jian-jun2   

  1. 1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,
    Tianjin 300134, China ;2. Tianjin Haihe Dairy Co. Ltd., Tianjin 300402, China
  • Received:2010-09-14 Online:2010-12-15 Published:2010-12-29
  • Contact: HU Zhi-he E-mail:hzhihe@tjcu.edu.cn

摘要:

利用胰蛋白酶水解酪蛋白,以水解物对瑞士乳杆菌增殖效果为指标,优化水解条件,并研究最佳增殖效果。 结果表明:胰蛋白酶水解酪蛋白的最佳水解条件为水解温度37℃、底物质量分数5%、酶与底物质量比1:600、pH8.0,此条件下制备的水解产物添加量为1g/100mL 时对瑞士乳杆菌生长增殖效果最好,而添加量大于4g/100mL 时,对瑞士乳杆菌生长呈现抑制作用。

关键词: 胰蛋白酶, 酪蛋白水解物, 瑞士乳杆菌, 增殖

Abstract:

Effect of casein hydrolysate on promoting the growth of Lactobacillus helvectucus was studied and the hydrolysis condition was optimized based on the growth promoting effect. The results showed that optimal hydrolysis conditions of casein by trypsin included temperature at 37 ℃, pH value 8.0, substrate concentration 5%, ratio of enzyme to substrate 1:600, and hydrolysis time 180 min. Casein hydrolysate prepared at this optimal condition was added into L. helvectucus culture solution, and the addition of 1 g/100mL casein hydrolysate exhibited the highest promoting effect on the growth of L. helveticus, while addition more than 4 g/100mL had the inhibiting effect.

Key words: rrypsin, casein hydrolyzate, Lactobacillus helvecticus, growth promoting

中图分类号: