食品科学 ›› 2010, Vol. 31 ›› Issue (23): 204-209.doi: 10.7506/spkx1002-6630-201023046

• 生物工程 • 上一篇    下一篇

白背毛木耳菌丝体深层发酵工艺优化

冀 宏,李 智,郑丽雪   

  1. 常熟理工学院生物与食品工程学院
  • 收稿日期:2010-08-24 修回日期:2010-11-13 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 冀宏 E-mail:jihong8848@126.com
  • 基金资助:

    2009 年江苏省科技厅苏北科技专项资助项目(BN2009320);苏州市科技发展计划项目(SZS201007)

Optimization of Submerged-fermentation Processing of Mycelia from Auricularia polytricha

JI Hong,LI Zhi,ZHENG Li-xue   

  1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2010-08-24 Revised:2010-11-13 Online:2010-12-15 Published:2010-12-29
  • Contact: JI Hong E-mail:jihong8848@126.com

摘要:

以菌丝体干质量浓度为考察指标,对白背毛木耳液体深层发酵的培养基及工艺条件进行优化。通过正交试验(L9(34))确定了发酵培养基最佳配方。在此基础上,基于三因素三水平的Box-Behnken 设计试验,采用响应曲面分析法对影响白背毛木耳菌丝体生长的发酵温度、摇瓶转速和发酵时间进行优化,确定最佳的培养条件。结果表明白背毛木耳深层发酵的最佳培养基组分为:葡萄糖2.00g/100mL、酵母膏0.20g/100mL、磷酸二氢钾0.25g/100mL、硫酸镁0.15g/100mL,当发酵温度为26℃、转速为182r/min、发酵时间为6.07d(145.7h)时发酵菌丝体干质量浓度最高,可达1.93g/100mL,较优化前提高了1.24 倍。

关键词: 白背毛木耳, 深层发酵, 菌丝体干质量, 正交试验, 响应曲面法

Abstract:

Biomass (dry weight) of mycelia was used as index to explore the optimal culture medium and processing conditions of submerged-fermentation of mycelia from Auricularia polytricha. The optimal fermentation broth formula was investigated by Box-Behnken and response surface analysis to be 2.00 g glucose, 0.20 g yeast extract, 0.25 g KH2PO4, and 0.15 g MgSO4 in 100mL of culture medium. The optimal fermentation conditions were fermentation temperature of 26 ℃, rotation speed of 182 r/min and fermentation time of 145.7 h. Under the optimal culture medium and fermentation conditions, the dry mycelia was up to 1.93 g in 100 mL of culture medium, which was increased by 1.24 fold compared with the conditions before optimization.

Key words: Auricularia polytricha, submerged-fermentation, biomass (dry weight) of mycelia, orthogonal experiment, response surface methodology

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