关键词: 蛹虫草, 虫草素, 氨基酸, 添加工艺

Abstract:

Histidine, arginine and lysine are alkaline amino acids that have an important effect on the growth of Cordyceps militaris. In this study, the effects of amino acid addition under various conditions on the growth of mycelium and the production of cordycepin as an anti-cancer functional ingredient during the fermentation process was investigated. Single factor experiments confirmed that the order of factors to affect the growth of mycelium during fermentation process from strong to weak was amino acid type, fermentation temperature, initial pH and amino acid amount. The optimal fermentation conditions for the growth of mycelium were explored by orthogonal experiments to be lysine as the selected amino acid, fermentation temperature of 26 ℃, initial pH of 6 and lysine amount of 0.3 g in 100 mL of medium. Similarly, single factor experiments confirmed that the order of factors to affect the production of cordycepin during fermentation process from strong to weak was amino acid type, initial pH, fermentation temperature and amino acid amount. The optimal fermentation conditions for the production of cordycepin were achieved by orthogonal experiments to be arginine as the selected amino acid, initial pH of 6, fermentation temperature of 24 ℃ and arginine amount of 0.3 g in 100 mL of medium.

Key words: Cordyceps militaris, cordycepin, amino acid, addition technology