食品科学
  
  
在线办公系统
文章快速检索  
  高级检索
ʳƷ��ѧ  2010, Vol. 31 Issue (23): 267-270    DOI:
���﹤�� ����Ŀ¼ | ����Ŀ¼ | ����� | �߼�����  |   
ˮ�����Ⱦ����Ǻϳ�ø����Ŀ�¡����˱��
����÷�������壬֣΢΢����ҫ��*
����ũҵ��ѧʳƷ��ѧ�빤��ѧԺ
Cloning and Eukaryotic Expression of Trehalose Synthase Gene from Thermus aquaticus FL-03
 ȫ��: PDF (511 KB)   HTML (0 KB)   ���: BibTeX | EndNote (RIS)      ��������
ժҪ ͨ����򹤳̼����ϳɺ����Ǻ�øTres ȫ���򣬲��뵽�ϳ��ĸ��������pPICZα�С���ת���ϳ��ĸ��GS115 �󣬾�Zeocin ����ɸѡ���Ծ��ꡣ��PCR ��ȫ��������Ŀ�Ļ���ı�ͨ���յ����Ŀ�ĵ��ף�����SDS-PAGE ��Western blotting ���м��ɹ��ع�����pPICZα-Tres ������������֤��Ŀ�Ļ���������ڱϳ��ĸ��Ļ����顣
����
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
E-mail Alert
RSS
�����������
����÷
�ؼ���� �����Ǻ�ø   Tres ȫ����   �����¡   ��˱��     
Abstract�� The full-length trehalose synthase gene (Tres) was synthesized by genetic engineering and inserted into the Pichia expression vector pPICZα. The expression vector carrying Tres gene was transformed into the competent cells of Pichia GS115 by means of electroporation, and Zeocin resistance screening was then performed to find positive strains. PCR and gene sequencing were used to identify the expression of the target gene. The expressed protein was analyzed by SDS-PAGE and Western blotting. The results suggested that the recombinant plamid pPICZα-Tres was successfully constructed and that the target gene had been integrated into the Pichia genome.
Key words�� trehalose synthase   Tres gene   gene cloning   eukaryotic expression   
�ո�����: 2010-09-14; ��������: 2010-12-29
ͨѶ����: ����÷     E-mail: spring430817@163.com;springljm@gmail.com
���ñ���:   
����÷. ˮ�����Ⱦ����Ǻϳ�ø����Ŀ�¡����˱��[J]. ʳƷ��ѧ, 2010, 31(23): 267-270.
LIU Jun-Mei. Cloning and Eukaryotic Expression of Trehalose Synthase Gene from Thermus aquaticus FL-03[J]. FOOD SCIENCE, 2010, 31(23): 267-270.
���ӱ���:  
http://124.205.222.100/Jwk_spkx/CN/     ��     http://124.205.222.100/Jwk_spkx/CN/Y2010/V31/I23/267
��
û�б��IJο�����
[1] ������1,2����С��3���� Ӱ3,*. ֬��ø�Ļ����¡�����з�������Ч��P��߻�����[J]. ʳƷ��ѧ, 2011, 32(11): 232-238.
版权所有 © 2010 《食品科学》编辑部
地址:北京市西城区禄长街头条4号《食品科学》编辑部 邮政编码:100050
总机:010-83155446/47/48/49/50 传真:010-83155436

本系统由 北京玛格泰克科技发展有限公司设计开发   技术支持:support@magtech.com.cn