关键词: 瑞士乳杆菌, 细胞因子, 肠道黏膜, 免疫应答

Abstract:

Our aim was to investigate the effects of Lactobacillus helveticus TS206 on intestinal immune response and cytokines in intestinal mucosa of healthy Balb/c mice. Mice were randomly assigned to three groups - normal control group, saline control group and L.helveticus group which were administered with the dose 108 CFU/mL. All the mice were executed after 0, 1, 2, 3, 4, 6, 8, 12, 24 h of administration and the SIgA and cytokines concentration were determined in intestine mucosa of all mice by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA). The results showed that compared with control group and physiological saline group, the SIgA content in intestinal mucosa of mice with the oral administration of L. helveticus was significant different (P＜0.01); IL-2 and IFN-γ content was also significant different (P＜0.05). IL-4 content in the intestinal mucosa of mice from treatment was significantly higher than control (P＜0.05), but not IL-6 expression level (P＞0.05). IFN-γ/IL-4 ratio exerted mutual antagonistic action, suggesting that L. helveticus maintain mucosa immunization balance in healthy mice, and also keep Th1/Th2 balance. Our results indicate that probiotic bacteria L.helveticus is provided with modulation of immune response in intestinal mucosa of mice.

Key words: Lactobacillus helveticus, cytokine, intestinal mucosa, mmune response