食品科学 ›› 2010, Vol. 31 ›› Issue (22): 1-5.doi: 10.7506/spkx1002-6630-201022001

• 工艺技术 •    下一篇

薏苡仁多糖的提取及分离纯化

肖小年1,曾海龙1,易 醒2,熊 华1,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室,中德联合研究院
    2.江西中德食品工程中心
  • 收稿日期:2010-06-29 修回日期:2010-10-13 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 熊华 E-mail:huaxiong100@hotmail.com
  • 基金资助:

    国家“863”计划项目(2008AA10Z332);江西省科技厅2008 年重点项目(2008S00594)

Isolation and Purification of Polysaccharides from Coix lachryma-jobi L. var. ma-yuen Stapf

XIAO Xiao-nian1,ZENG Hai-long1,YI Xing2,XIONG Hua1,*   

  1. 1. State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Nanchang
    330047, China;2. Jiangxi Sino-German Food Engineering Center, Nanchang 330047, China
  • Received:2010-06-29 Revised:2010-10-13 Online:2010-11-25 Published:2010-12-29
  • Contact: XIONG Hua E-mail:huaxiong100@hotmail.com

摘要:

在单因素试验基础上,选取料液比、提取温度和提取时间3 项为考察因素,通过正交试验L9(33)优化薏苡仁多糖提取的最佳工艺条件;对薏苡仁多糖进行分离纯化,考察薏苡仁多糖的SephadexG-75 凝胶柱层析特性以及纯度鉴定。结果表明:料液比1:15(g/mL)、提取温度100℃、提取时间150min 为最佳提取条件,在最佳条件下薏苡仁多糖得率为5.53%;紫外扫描结果显示薏苡仁多糖中不含核酸和蛋白质。

关键词: 薏苡仁多糖, 正交试验, 提取, 分离纯化

Abstract:

In order to maximize the extraction yield of polysaccharides from the seeds of adlay (Coix lachryma-jobi L. var. mayuen Stapf), an orthogonal array design L9(33) was applied to optimize the extraction conditions including raw material/ water as well as extraction temperature and duration, of which the effects on polysaccharide extraction yield were initially dealt with by single factor method. Finally, the extracted polysaccharides were purified on Sephadex G-75 gel column, and the chromatographic characteristics were investigated and purity identification was carried out. Material/water ratio of 1:15 (g/mL), extraction temperature of 100 ℃ and extraction time of 150 min were found optimal, and the extraction yield of polysaccharides was 5.53% under these optimal extraction conditions. The purified polysaccharides were analyzed by UV spectroscopy to contain neither protein nor nucleic acids.

Key words: coixan, orthogonal array design, extraction, isolation and purification

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