食品科学 ›› 2010, Vol. 31 ›› Issue (22): 75-80.doi: 10.7506/spkx1002-6630-201022016

• 工艺技术 • 上一篇    下一篇

水酶法提取斑点叉尾鮰内脏油的工艺研究

陆剑锋,林 琳,张伟伟,翁世兵,叶应旺,姜绍通   

  1. 合肥工业大学生物与食品工程学院
  • 收稿日期:2010-07-19 修回日期:2010-10-22 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 陆剑锋 E-mail:lujf@sibs.ac.cn
  • 基金资助:

    安徽省重大科技攻关专项(08010301078);国家星火计划项目(2008GA710021)

Aqueous Enzymatic Extraction of Fish Oil from Viscera of Channel Catfish (Ictalurus punctatus)

LU Jian-feng,LIN Lin,ZHANG Wei-wei,WENG Shi-bing,YE Ying-wang,JIANG Shao-tong   

  1. (College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
  • Received:2010-07-19 Revised:2010-10-22 Online:2010-11-25 Published:2010-12-29
  • Contact: LU Jian-feng E-mail:lujf@sibs.ac.cn

摘要:

为提高鱼类加工废弃物的综合利用率,以斑点叉尾鮰内脏为原料,采用水酶法制备鱼油。结果表明:水酶法提取鱼油的最佳工艺条件为初始pH7.5、料液比1:1、加酶量3500U/g、酶解温度55℃、酶解时间0.5h,该条件下鱼油的提取率达到80.94%;提取到的鱼油(即粗鱼油)外观呈黄褐色,具有鱼腥味和轻微的酸败味,其过氧化值较低,而酸值相对较高;粗鱼油中的饱和脂肪酸相对含量为19.42%,单不饱和脂肪酸和多不饱和脂肪酸分别为61.76% 和16.83%,Σn-3/Σn-6 的脂肪酸比值达到3.63。表明斑点叉尾鮰内脏是生产鱼油的良好来源。

关键词: 斑点叉尾鮰, 内脏, 鱼油, 水酶法

Abstract:

Fish oil was extracted from the viscera of channel catfish (Ictalurus punctatus) by aqueous enzymatic extraction method. The optimum extraction parameters were as follows: initial hydrolysis pH 7.5, ratio of viscera to water 1:1, amount of papain 3500 U/g, hydrolysis temperature 55 ℃, hydrolysis time 0.5 h. Under these conditions, the extraction ratio of fish oil was up to 80.94%. The extracted fish oil (namely crude fish oil) was yellow-brown in appearance with fishy smell and light rancidity odor, and it had a lower peroxide value and high acid value. The relative content of the saturated fatty acids accounted for 19.42% of the total fatty acids. The relative content of monounsaturated fatty acids and polyunsaturated fatty acids were 61.76% and 16.83%, respectively. The ratio of n-3 to n-6 polyunsaturated fatty acids is 3.63. The above results show that the viscera of channel catfish are a good resource for fish oil production.

Key words: Ictalurus punctatus, viscera, fish oil, aqueous enzymatic extraction method

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