食品科学 ›› 2010, Vol. 31 ›› Issue (22): 96-100.doi: 10.7506/spkx1002-6630-201022020

• 工艺技术 • 上一篇    下一篇

响应面法优化碱法提取林蛙卵团中粗多糖的工艺研究

刘晓丹,郑明珠,刘景圣*   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2010-05-26 修回日期:2010-10-08 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 刘景圣 E-mail:liujs1007@vip.sina.com.cn
  • 基金资助:

    国家自然科学基金项目(30671536)

Optimization of Alkaline Extraction Conditions for Crude Polysaccharides from Rana Egg Jelly by Response Surface Methodology

LIU Xiao-dan,ZHENG Ming-zhu,LIU Jing-sheng*   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2010-05-26 Revised:2010-10-08 Online:2010-11-25 Published:2010-12-29
  • Contact: LIU Jing-sheng E-mail:liujs1007@vip.sina.com.cn

摘要:

以林蛙卵团胶状物为原料,采用碱法对粗多糖进行提取,在单因素试验基础上,采用响应面法对NaOH溶液浓度、料液比、提取温度及提取时间进行优化。结果表明,碱法提取林蛙卵团胶状物粗多糖的最佳工艺条件为NaOH 溶液浓度1.2mol/L、料液比1:8(g/mL)、浸提温度56℃、浸提时间73min。在此条件下林蛙卵团胶状物粗多糖含量可达24.01mg/g。

关键词: 林蛙, 卵团胶状物, 多糖, 响应面

Abstract:

Rana egg jelly was used as the raw material to extract polysaccharides by an alkaline extraction method. Based on single factor experiments, NaOH concentration, material-liquid ratio, extraction temperature and extraction time during extraction process were optimized by response surface methodology. Results exhibited the best extraction conditions were NaOH concentration of 1.2 mol/L, material-liquid ratio of 1: 8, extraction temperature of 56 ℃ and extraction time of 73 min. The extraction rate of crude polysaccharides can reach up to 24.01 mg/g.

Key words: Rana;egg jelly, polysaccharides, response surface methodology

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