关键词: 骨胶原蛋白, 胰蛋白酶, 水解度, 酶解工艺

Abstract:

Collagen was used as the material to prepare collagen polypeptide through enzymatic hydrolysis by trypsin. The optimal hydrolysis conditions were explored by single factor and response surface experiments on the basis of hydrolysis degree. Results indicated that the optimal hydrolysis conditions were hydrolysis pH of 6.91, hydrolysis temperature of 60 ℃, enzyme dosage of 200 mg per 1 g collagen, material-liquid ratio of 1: 615 (g/mL), and hydrolysis time of 4 h. Under the optimal hydrolysis conditions, the hydrolysis degree of collagen was 29.36%. The solubility and emulsification capability of hydrolyzed collagen were significantly improved.

Key words: bone collagen, trypsin, the degree of hydrolysis, enzymatic hydrolysis processing