食品科学 ›› 2010, Vol. 31 ›› Issue (22): 160-163.doi: 10.7506/spkx1002-6630-201022034

• 工艺技术 • 上一篇    下一篇

匙吻鲟软骨蛋白酶解工艺优化

吴 越1,2,3,沈 硕1,3,熊善柏1,2,3,赵思明1,2,3,黄琪琳1,2,3,*   

  1. 1.华中农业大学食品科学技术学院
    2. 国家大宗淡水鱼加工技术研发分中心 3.湖北省水产品加工工程技术研究中心
  • 收稿日期:2010-07-01 修回日期:2010-10-22 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 黄琪琳 E-mail:hql@mail.hzau.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD30B01)

Optimal Enzymatic Degradation of Cartilage Protein from Polyodon spathula

WU Yue1,2,3,SHEN Shuo1,3,XIONG Shan-bai1,2,3,ZHAO Si-ming1,2,3,HUANG Qi-lin1,2,3   

  1. 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. National R & D Branch Center for Conventional Freshwater Fish Processing, Wuhan 430070, China ;
    3. Aquatic Product Engineering and Technology Research Center of Hubei Province, Wuhan 430070, China
  • Received:2010-07-01 Revised:2010-10-22 Online:2010-11-25 Published:2010-12-29
  • Contact: HUANG Qi-lin E-mail:hql@mail.hzau.edu.cn

摘要:

为提高匙吻鲟软骨蛋白的利用价值和生物活性,以匙吻鲟软骨为原料,经微波- 碱法提取软骨蛋白,通过单因素试验确定碱性蛋白酶酶解软骨蛋白的关键因素为酶解温度、底物质量分数、pH 值和酶的用量。经正交试验优化,确定了酶解的最佳工艺条件。结果表明:最佳酶解工艺条件为酶解温度50℃、软骨蛋白质量分数1%、pH7.5、酶用量9000U、酶解时间5h,在此条件下氨基酸态氮生成率最高,达到21.89%。

关键词: 匙吻鲟, 软骨蛋白, 酶解

Abstract:

In order to improve the utilization value and biological activity of cartilage protein, Polyodon spathula cartilage was used as the material to extract cartilage protein by microwave-alkali extraction method. The optimal enzymatic hydrolysis conditions of cartilage protein were explored by single factor and orthogonal experiments to be enzymatic hydrolysis temperature of 50 ℃, cartilage protein concentration of 1%, hydrolysis pH of 7.5, alkaline protease amount of 9000 U, and hydrolysis time of 5 h. The yield of amino nitrogen was 21.89% under the optimal conditions.

Key words: Polyodon spathula, cartilage protein, enzymatic hydrolysis

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