关键词: 酶法, 米糠蛋白, 提取, 碱法

Abstract:

Rice bran was used as the raw material to extract rice bran protein by alkali extraction method and enzymatic hydrolysis method. The optimal extraction processing parameters were explored by orthogonal experiments through evaluating the effects of material-liquid ratio, enzyme addition amount, extraction time and extraction temperature on extraction rate of rice bran protein. Results indicated that the optimal extraction processing parameters for alkali extraction method were material-liquid ratio of 1:10, extraction time of 2.5 h, and extraction temperature of 45 ℃. The extraction rate of rice bran protein was 57.8% under the optimal extraction conditions. Similarly, the optimal extraction processing parameters for enzymatic hydrolysis method were enzyme dosage of 2% (E/S), hydrolysis time of 2.5 h, hydrolysis temperature of 50 ℃. The extraction rate of rice bran protein was 38.4% under the optimal hydrolysis conditions. These studies will provide a theoretical reference for protein extraction from rice bran.

Key words: rice bran protein, extraction, alkali extraction metod, enzymatic hydrolysis method