食品科学 ›› 2010, Vol. 31 ›› Issue (22): 268-272.doi: 10.7506/spkx1002-6630-201022057

• 工艺技术 • 上一篇    下一篇

响应面法优化无梗五加果多糖超声波、微波法提取工艺研究

冯 颖1,陈巧红1,孟宪军1,王建国2   

  1. 1.沈阳农业大学食品学院 2.辽宁省质量技术监督局
  • 收稿日期:2010-06-20 修回日期:2010-11-09 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 冯颖 E-mail:fywjg@sina.com
  • 基金资助:

    辽宁省博士科研启动基金项目(20091068)

Optimization of Microwave and Ultrasonic Extraction of Polysaccharides from Acanthopanax sessiliflorus Fruits by Response Surface Methodology

FENG Ying1,CHEN Qiao-hong1,MENG Xian-jun1,WANG Jian-guo2   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. Liaoning Bureau of Quality and Technical Supervision, Shenyang 110003, China
  • Received:2010-06-20 Revised:2010-11-09 Online:2010-11-25 Published:2010-12-29
  • Contact: FENG Ying E-mail:fywjg@sina.com

摘要:

根据中心组合(Box-Behnken)试验设计原理采用响应面分析法,分别对无梗五加果多糖的超声波提取工艺和微波提取工艺进行优化。结果表明:超声波法提取多糖的最佳工艺参数为料液比1:70(g/mL)、提取时间50min、提取功率175W、提取温度为60℃,实际测得多糖得率为3.491%;微波法提取多糖的最佳工艺参数为料液比1:40(g/mL)、提取时间80s、提取功率595W,实际测得多糖得率为3.482%,与模型预测值基本相符。

关键词: 无梗五加, 多糖, 提取, 响应面

Abstract:

The response surface methodology based on Box-Behnken design principle was used to optimize processing parameters of ultrasonic and microwave extraction for polysaccharides from Acanthopanax sessiliflorus fruits. Results showed that the optimal processing conditions of ultrasonic extraction were material-liquid ratio of 1:70 (g/mL), ultrasonic power of 175W, extraction temperature of 60 ℃ and extraction time of 50 min. The optimal processing conditions of microwave extraction were material-liquid ratio of 1:40 (g/mL), microwave power of 595 W and extraction time of 80 s. The yields of polysaccharides were up to 3.491% and 3.482% under the optimal ultrasonic and microwave extraction conditions, respectively, which was consistent with the predicted results.

Key words: Acanthopanax sessiliflorus, polysaccharide, extraction, response surface methodology

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