食品科学 ›› 2010, Vol. 31 ›› Issue (22): 297-300.doi: 10.7506/spkx1002-6630-201022064

• 工艺技术 • 上一篇    下一篇

马铃薯分离蛋白的提取工艺

齐 斌1,2,郑丽雪1,朴金苗1   

  1. 1.常熟理工学院生物与食品工程学院 2.苏州市食品生物技术重点实验室
  • 收稿日期:2010-06-28 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 齐斌 E-mail:qibin64@126.com

Extraction Processing of Potato Protein Isolate

QI Bin1,2,ZHENG Li-xue1,PIAO Jin-miao1   

  1. 1. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China;
    2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China
  • Received:2010-06-28 Online:2010-11-25 Published:2010-12-29
  • Contact: QI Bin1 E-mail:qibin64@126.com

摘要:

采用盐溶碱提酸沉法制备马铃薯分离蛋白,并对马铃薯分离蛋白提取条件和功能特性进行研究。十二烷基硫酸钠- 聚丙烯酰胺电泳结果表明,马铃薯分离蛋白的亚基相对分子质量较广,其中分子质量低于21.0kD 的亚基较多。马铃薯分离蛋白的最佳提取工艺为料水比1:10(g/mL)、温度40℃、pH8.0、NaCl 浓度0.4mol/L。

关键词: 马铃薯, 分离蛋白, 十二烷基硫酸钠-聚丙烯酰胺电泳

Abstract:

Potato protein isolate (PPI) was prepared from fresh potato by salt-soluble alkali extraction and acid precipitation. Extraction conditions and functional properties of PPI were investigated. SDS-PAGE revealed that PPI was composed of proteins with a broad range of molecular weights, especially rich in proteins with small molecular weight less than 21.0 kD. Meanwhile, the optimal extraction conditions were material-liquid ratio of 1:10 (g/mL), extraction temperature of 40 ℃, pH 8.0, and NaCl concentration of 0.4 mol/L.

Key words: potato, protein isolate, SDS-PAGE

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