食品科学 ›› 2010, Vol. 31 ›› Issue (21): 226-231.doi: 10.7506/spkx1002-6630-201021051

• 生物工程 • 上一篇    下一篇

液态发酵法制备菜籽ACE抑制肽培养基条件优化

王立峰1,2,金 晶1,袁 建1,何 荣1,2,鞠兴荣1   

  1. 1.南京财经大学食品科学与工程学院,江苏省粮油品质控制及深加工技术重点实验室
    2.江南大学食品学院
  • 收稿日期:2010-01-11 修回日期:2010-07-27 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 王立峰 E-mail:wanglifeng_8@163.com
  • 基金资助:

    江苏省自然科学基金项目(BK2010573);江苏省农业科技自主创新资金项目(cx(10)444);
    国家农业成果转化基金项目(2009C10045)

Optimization of Liquid-state Fermentation Conditions for Preparing Rapeseed Angiotensin I-converting Enzyme Inhibitory Peptides

WANG Li-feng1,2,JIN Jing1,YUAN Jian1,HE Rong1,2,JU Xing-rong1   

  1. (1. Jiangsu Province Key Laboratory of Grain and Oils Quality Control and Deep-Utilizing Technology, College of Food Science and
    Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China;2. School of Food Science and Technology,
    Jiangnan University, Wuxi 214122, China)
  • Received:2010-01-11 Revised:2010-07-27 Online:2010-11-15 Published:2010-12-29
  • Contact: Li-Feng WANG E-mail:wanglifeng_8@163.com

摘要:

以菜籽粕为原料,通过枯草芽孢杆菌液态发酵生产菜籽ACE 抑制肽。先以肽得率、ACE 抑制率为指标通过单因素试验得到液态发酵的培养基条件,再以响应面分析法,优化枯草芽孢杆菌液态发酵培养基中的3 种成分,确定枯草芽孢杆菌发酵生产菜籽肽的最佳培养基工艺条件为:磷酸二氢钾0.45g/100mL、葡萄糖0.8g/100mL、料液比1:23、初始pH7.0。优化后的ACE 抑制率可达69.79%。

关键词: 菜籽肽, 液态发酵, ACE抑制肽, 响应面分析法

Abstract:

Rapeseed meal was used to prepare angiotensin I-converting enzyme (ACE) inhibitory peptide through liquidstate fermentation by Bacillus subtilis. The optimal fermentation conditions were explored by single factor and response surface experiments through evaluating the yield of peptide and ACE inhibitory rate. The optimal fermentation conditions were 0.45 g/100mL potassium dihydrogen phosphate, 0.8 g/100mL glucose, material/liquid ratio of 1: 23 and initial pH 7.0. The ACE inhibitory rate reached up to 69.79% under these optimal fermentation conditions.

Key words: rapeseed peptide, liquid-state fermentation, ACE inhibitory peptide, response surface methodology

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