食品科学 ›› 2010, Vol. 31 ›› Issue (20): 43-46.doi: 10.7506/spkx1002-6630-201020009

• 工艺技术 • 上一篇    下一篇

膜技术分离纯化金银花绿原酸提取液工艺研究

范远景1,马凌云1,徐晓伟1,张 平2,方桐辉2,胡晓薇2   

  1. 1. 合肥工业大学生物与食品工程学院 2.安徽省祁门黄山药谷科技有限公司
  • 收稿日期:2010-02-08 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 范远景 E-mail:swf89105@mail.hf.ah.cn
  • 基金资助:

    黄山市重点科技项目(2009ZN-09)

Application of Membrane Separation for the Purification of Chlorogenic Acid-rich Extract from Lonicera japonica Thunb.

FAN Yuan-jing1,MA Ling-yun1,XU Xiao-wei1, ZHANG Ping2,FANG Tong-hui2,HU Xiao-wei2   

  1. 1. College of Biotechnology and Food Engineeing, Hefei University of Technology, Hefei 230009, China;
    2. Anhui Huangshan Herb Valley Bio-Technology Co. Ltd., Qimen 245600, China
  • Received:2010-02-08 Online:2010-10-25 Published:2010-12-29
  • Contact: FAN Yuan-jing1 E-mail:swf89105@mail.hf.ah.cn

摘要:

研究膜技术分离纯化绿原酸提取液的过程。以金银花为原料,以平均通量、膜截留率、绿原酸透过率为指标,比较3 种微滤膜MF1、MF2 和MF3,4 种超滤膜UF1、UF2、UF3 和UF4,及两种反渗透膜RO1 和RO2 对绿原酸提取液的过滤特性,并研究浓缩液的洗滤对绿原酸截留率的影响。结果表明:MF1、UF1 和RO2膜的过滤性能明显优于其他同类型膜,使用MF1-UF1-RO2 膜组合处理效果最佳,其绿原酸回收率可达67.75%,产品纯度可达13.21% 以上;同时,洗滤可进一步提高处理效果,经间歇洗滤后,MF1 膜的绿原酸截留率可由9.54% 降低到1.17%,UF2 膜的绿原酸截留率可由32.36% 降低到20.11%。

关键词: 金银花, 绿原酸, 膜技术, 超滤

Abstract:

In terms of average membrane permeation flux, membrane retention rate and permeation rate of chlorogenic acid, the applications of three microfiltration membranes (MF1, MF2 and MF3), four ultrafiltration membranes (UF1, UF2, UF3 and UF4) and two reverse osmosis membranes (RO1 and RO2) for the purification of the chlorogenic acid-rich extract from Lonicera japonica Thunb were evaluated. MF1, UF1 and RO2 were found to be even better than other similar membranes. Optimal purification was achieved by the sequential combined use of MF1, UF1 and RO2, and the maximum chlorogenic acid recovery was up to 67.75%, and a product with a purity exceeding 13.21% was obtained. Additionally, diafiltration could improve the treatment effect, and the retention rate of chlorogenic acid was decreased from 9.54% to 1.17% in MF1 separation and from 32.36% to 20.11% in UF2 separation after intermittent diafiltration.

Key words: Lonicera japonica Thunb, chlorogenic acid, membrane technology, ultrafiltration

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