食品科学 ›› 2010, Vol. 31 ›› Issue (20): 127-131.doi: 10.7506/spkx1002-6630-201020026

• 工艺技术 • 上一篇    下一篇

高速逆流色谱分离制备茶粕中茶皂素单体

陈 力,邓泽元,胡蒋宁* ,李 静,范亚苇,刘 蓉   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2010-06-27 修回日期:2010-09-09 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 胡蒋宁 E-mail:hujiangning2005@hotmail.com
  • 基金资助:

    2010 年江西省重大科技专项

Isolation and Preparation of Teasaponin Monomers from Tea Seed Cake by High-Speed Counter-current Chromatography

CHEN Li,DENG Ze-yuan,HU Jiang-ning*,LI Jing,FAN Ya-wei,LIU Rong   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2010-06-27 Revised:2010-09-09 Online:2010-10-25 Published:2010-12-29
  • Contact: HU Jiang-ning E-mail:hujiangning2005@hotmail.com

摘要:

目的:建立一种高效、快速的分离制备茶皂素单体的高速逆流色谱方法。方法:微波辅助提取茶皂素后,用D-101 大孔树脂初步纯化,所得粗品经高速逆流色谱分离纯化,乙酸乙酯- 正丁醇- 水(1:4:4,V/V,含体积分数3% 的乙酸)为两相溶剂系统,转速800r/min、流速1.5mL/min、检测波长267nm、进样量100mg,所得分离收集液经高效液相色谱法检测。结果:从茶皂素粗提物中分离得到纯度分别为99.1% 和94.5% 的两种茶皂素单体,经干燥称得其质量分别为11mg 和15mg。结论:该方法制备茶皂素单体简便、快速,所得产物的纯度高,为茶皂素的分离纯化提供了一种新途径。

关键词: 高速逆流色谱(HSCCC), 茶皂素单体, 制备, 纯化

Abstract:

Objective: To develop a rapid and efficient method for preparing teasaponin monomers from tea seed cake using preparative high-speed counter-current chromatography (HSCCC). Methods: A crude extract rich in teasaponin monomers was obtained from tea seed cake by microwave-assisted extraction, and preliminarily purified by D-101 macroporous resin chromatography, followed by further HSCCC purification/fractionation with a binary phase system made up of ethyl acetate, n-butanol and 3% acetate (1:4:4, V/V) at a flow rate of 1.5 mL/min with rotation at 800 r/min under the conditions of 267 nm detection wavelength and 100 mg sample load. The final purification product was analyzed by HPLC. Results: Two teasaponin monomers with 99.1% and 94.5% purities were obtained after the HSCCC separation, and their dry weights were 11 mg and 15 mg, respectively. Conclusion: The proposed HSCCC method has the characteristics of simplicity, rapidity and high product purity, thereby providing a novel strategy for the separation of teasaponin monomers.

Key words: high-speed counter-current chromatography (HSCCC), teasaponin monomers, preparation, purification

中图分类号: