食品科学 ›› 2010, Vol. 31 ›› Issue (20): 152-158.doi: 10.7506/spkx1002-6630-201020031

• 工艺技术 • 上一篇    下一篇

超声波技术在波纹巴非蛤酶解反应中的应用

陈纯馨1,孙恢礼2,吴嘉辉1,陈 忻1,陈智刚3,陈晓刚1   

  1. 1.佛山科学技术学院理学院 2.中国科学院南海海洋研究所 3.安安美容保健品有限公司
  • 收稿日期:2010-06-25 修回日期:2010-09-14 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 陈晓刚 E-mail:fscxg@189.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2008BAD94B08);中国科学院知识创新工程重要方向项目(KZCX2-YW-209);
    广东省 2009 院省合作项目(00617381220824077)

Application of Ultrasonic Technology to the Enzymatic Hydrolysis of Paphia undulata Muscle

CHEN Chun-xin1,SUN Hui-li2,WU Jia-hui1,CHEN Xin1,CHEN Zhi-gang3,CHEN Xiao-gang1   

  1. 1. School of Science, Foshan University, Foshan 528000, China;
    2. South China Sea Institute of Oceanology, China Academy of Sciences, Guangzhou 510301, China;
    3. Anan Beauty and Health Products Co. Ltd., Foshan 528000, China
  • Received:2010-06-25 Revised:2010-09-14 Online:2010-10-25 Published:2010-12-29
  • Contact: CHEN Xiao-gang1 E-mail:fscxg@189.cn

摘要:

将超声波技术应用到波纹巴非蛤制备的酶解反应中,在单因素试验的基础上采用响应面分析法对试验条件进行优化。运用Box-Benhnken 的中心组合试验设计原理,选择对短肽产率显著影响的4 个因素:辐照时间、辐照温度、超声功率和固液比,进行四因素五水平的响应面分析试验,确定在超声波条件下波纹巴非蛤的酶解反应最佳工艺条件为辐照时间4h、辐照温度55℃、超声功率140W、固液比1:2(g/mL)。对模型预测结果进行验证,得到短肽产率为样品的5.15%,是无超声波作用下的短肽产率的1.1 倍。

关键词: 超声波技术, 波纹巴非蛤, 酶解, 优化, 响应面分析法

Abstract:

The present study was aimed at optimizing the ultrasonic-assisted enzymatic hydrolysis of Paphia undulata muscle for producing short-chain peptides using response surface analysis based on a series of single-factor investigations. By means of the principles of Box-Benheken central composite experimental design, a four-variable, five-level response surface analysis was designed to discuss the effects of ultrasonic treatment time and power, hydrolysis temperature and solid/liquid ratio on shortchain peptide production. The optimal levels of these four process conditions were determined as follows: ultrasonic treatment time, 4 h; hydrolysis temperature, 55 ℃; ultrasonic power, 140 W; and solid/liquid ratio, 1:2 (g/mL). The experimental value of peptide yield was 5.15%, 1.1-fold higher than that obtained without ultrasonic assistance.

Key words: ultrasonic technology, Paphia undulata, enzymolysis, optimization, response surface methodology

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