食品科学 ›› 2010, Vol. 31 ›› Issue (19): 79-83.doi: 10.7506/spkx1002-6630-201019015

• 基础研究 • 上一篇    下一篇

泰和乌骨鸡活性肽的分离及其体外抗氧化作用

刘建华,田颖刚,王 勇,朱 胜,王春艳,张 盼,谢明勇*   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2010-06-19 修回日期:2010-09-09 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 谢明勇 E-mail:myxie@ncu.edu.cn
  • 基金资助:

    国家自然科学基金项目(20862012)

Isolation and in vitro Antioxidant Activity of Active Peptides from Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson) Muscle

LIU Jian-hua,TIAN Ying-gang,WANG Yong,ZHU Sheng,WANG Chun-yan,ZHANG Pan,XIE Ming-yong*   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2010-06-19 Revised:2010-09-09 Online:2010-10-15 Published:2010-12-29
  • Contact: XIE Ming-yong* E-mail:myxie@ncu.edu.cn

摘要:

利用制备型HPLC 对泰和乌骨鸡活性肽进行分离,流动相为0.01mol/L 的磷酸盐溶液,采用强酸型离子交换树脂和强碱型离子交换树脂两步脱盐法对泰和乌骨鸡活性肽各分离组分进行脱盐处理。以肌肽和抗坏血酸为对照,通过清除羟自由基作用、抑制脂质过氧化作用以及对Fe2+ 和Cu2+ 螯合能力4 个体系测定泰和乌骨鸡活性肽及其分离组分的体外抗氧化能力。结果表明:泰和乌骨鸡活性肽分离所得13 个组分均具有一定的抗氧化能力,且与质量浓度呈量效关系,绝大部分分离活性肽组分比活性总肽有更强的羟自由基清除能力、脂质过氧化抑制能力以及Fe2+ 螯合能力。

关键词: 泰和乌骨鸡活性肽, 抗氧化, 清除自由基, 抑制脂质过氧化, 金属螯合能力

Abstract:

The Taihe black-bone silky fowl muscle hydrolysate prepared by papain hydrolysis were separated to obtain active peptides by preparative HPLC with 0.01 mol/L phosphate buffer as the mobile phase, and the separated fractions were then pooled and desalted stepwise by 001 × 16 strong acid cation exchange resin and 201 × 7 strong basic anion exchange resin column chromatography. The hydrolysate and its separated fractions were tested for their in vitro antioxidant effects by hydroxyl free radical scavenging array, lipid peroxidation inhibition assay and measuring the ability to chelate Fe2+ and Cu2+ and compared with carnosine and ascorbic acid. Totally 13 fractions were separated from the Taihe black-bone silky fowl muscle hydrolysate, and all of them had obvious antioxidant effect in a concentration-dependent way. Most of the fractions presented stronger abilities to scavenge hydroxyl free radicals, inhibit lipid peroxidation and chelate Fe2+ than the hydrolysate.

Key words: active peptides derived from Taihe black-bone silky fowl muscle, antioxidation, free radical scavenging ability, peroxidation inhibition, metal chelating ability

中图分类号: