食品科学 ›› 2010, Vol. 31 ›› Issue (19): 92-95.doi: 10.7506/spkx1002-6630-201019018

• 基础研究 • 上一篇    下一篇

氮元素对发菜生长及多糖含量的影响

汤 俊1,2,万 能2,胡征宇1,*   

  1. 1.中国科学院水生生物研究所 2.常熟理工学院生物与食品工程系
  • 收稿日期:2010-02-24 修回日期:2010-09-17 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 胡征宇 E-mail:Huzy@ihb.ac.cn
  • 基金资助:

    国家自然科学基金项目(30671611);苏州市科技局应用藻类学重点实验室基金项目(SZS0806)

Effect of Nitrogen on the Growth and Polysaccharide Content of Nostoc flagelliforme

TANG Jun1,2,WAN Neng2,HU Zheng-yu1,*   

  1. 1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
    2. Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2010-02-24 Revised:2010-09-17 Online:2010-10-15 Published:2010-12-29
  • Contact: HU Zheng-yu1 E-mail:Huzy@ihb.ac.cn

摘要:

为优化培养条件,研究含氮(BG11)和无氮(BG110)培养基对发菜(Nostoc flagelliforme)的色素、藻胆蛋白及胞内多糖和胞外多糖(EPS)含量的影响。结果显示:含氮组发菜的叶绿素a(Chl a)、类胡萝卜素(Carotenoids)、藻蓝蛋白(PC)、别藻蓝蛋白(APC)、藻红蛋白(PE)的含量均显著高于无氮组;胞内及胞外多糖含量无氮组高于含氮组,两者胞内多糖含量最大分别为1.03mg/mL 和0.78mg/mL,EPS 含量最大分别为243.8μg/mL 和74.9μg/mL。表明氮元素的缺乏对发菜的色素合成有抑制作用,但在一定程度上能够促进胞内及胞外多糖的合成和分泌。

关键词: 发菜, 氮, 多糖, 藻胆蛋白

Abstract:

Cultivation of Nostoc flagelliforme in nitrogen-containing (BG11) and non-nitrogen-containing (BG110) media was conducted for figuring out the effects of nitrogen on the growth and the contents of phycobiliprotein (PC), allophycocyanin (APC), phycoerythrin (PE) and endo- and exopolysaccharides of Nostoc flagelliforme were investigated. The results showed that after 20 d cultivation, compared to BG110 medium, N. flagelliforme cultured in BG11 medium had much higher contents of Chl a, carotenoides, PE, APC and PC, but much lower contents of endo- and exo polysaccharides. The maximum content of endopolysaccharides was 1.03 mg/mL in BG110 and 0.78 mg/mL in BG11, while that of exopolysaccharides was 243.8μg/mL in BG110 and 74.9μg/mL in BG11. These results indicate that nitrogen deficiency may inhibit the growth of N.flagelliforme, but promote the synthesis of endo- and exopolysaccharides to a certain extent.

Key words: Nostoc flagelliforme, nitrogen, polysaccharide, phycobiliprotein

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