食品科学 ›› 2010, Vol. 31 ›› Issue (19): 313-316.doi: 10.7506/spkx1002-6630-201019068

• 生物工程 • 上一篇    下一篇

沙城龙眼葡萄园土壤中酵母菌多样性研究

李 艳1,2,卢 君1,张利中3   

  1. 1.河北科技大学生物科学与工程学院
    2.河北省发酵工程技术研究中心
    3.中国长城葡萄酒有限公司
  • 收稿日期:2010-06-01 修回日期:2010-09-26 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 李艳 E-mail:lymdh5885@163.com
  • 基金资助:

    河北省2009 年科技支撑计划项目(092210003D)

Biodiversity of Yeast in Soils from Longan Grape Plantations in Shacheng Region

LI Yan1,2,LU Jun1,ZHANG Li-zhong3   

  1. 1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China;
    2. R&D Center for Fermentation Engineering of Hebei Province, Shijiazhuang 050018, China;
    3. China Great Wall Wine Co. Ltd., Shacheng 075400, China)
  • Received:2010-06-01 Revised:2010-09-26 Online:2010-10-15 Published:2010-12-29
  • Contact: LI Yan E-mail:lymdh5885@163.com

摘要:

为分离筛选具有产区特色的菌种,本实验采用梯度稀释和划线分离技术,从沙城地区3 个龙眼葡萄园土壤中共分离得到210 株酵母菌。按照形态表型分为9 类,又利用核糖体5.8S rDNA-ITS 区域RFLP 方法将这9 类酵母在分子水平上进行了区分,其中两类酵母为常见酵母,分别为东方伊萨酵母(Issatchenkia orientalis)和热带假丝酵母(Candida tropicalis)。实验证明了该地区土壤中酵母菌的多样性。

关键词: 龙眼葡萄, 土壤, 酵母, 形态表型, RFLP 方法, 生物多样性

Abstract:

To isolate and screen characteristic yeast strains, 210 yeast strains were isolated from soils from three Longan grape plantations in Shacheng region by gradient dilution and streak plate methods. These stains were classified into nine groups according to their morphological phenotypes, and the nine groups were identified by 5.8S ITS restriction fragment length polymorphism (RFLP) analysis, and two of them, Issatchenkia orientalis and Candida tropicalis, were common yeast strains. This study confirms the biodiversity of yeast in the oil of this region.

Key words: Longan vineyard, soil, yeast, morphological phenotypes RFLP method, biodiversity

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