食品科学 ›› 2010, Vol. 31 ›› Issue (19): 317-320.doi: 10.7506/spkx1002-6630-201019069

• 营养卫生 • 上一篇    下一篇

苦荞麦蛋白质对乳腺癌细胞的增殖抑制作用

郭晓娜,姚惠源   

  1. 江南大学食品学院
  • 收稿日期:2010-01-21 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 郭晓娜 E-mail:gxn1978@hotmail.com
  • 基金资助:

    国家自然科学基金项目(30900999);高等学校博士学科点专项科研基金项目(200802951003)

Anti-proliferative Effect of Tartary Buckwheat Protein Fraction TBWSP31 on Breast Cancer Cells

GUO Xiao-na,YAO Hui-yuan   

  1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Received:2010-01-21 Online:2010-10-15 Published:2010-12-29
  • Contact: GUO Xiao-na E-mail:gxn1978@hotmail.com

摘要:

采用MTT 法、HE 染色法、扫描电镜法研究苦荞麦蛋白质(TBWSP31)对人乳腺癌Bcap37 细胞的增殖抑制作用。结果表明:TBWSP31 对人乳腺癌细胞株Bcap37 的生长有明显的抑制作用,并且存在时间效应和剂量效应。48h 和72h 的IC50 值分别为43.37、19.75μg/mL。HE 染色发现细胞经样品作用后,细胞变形,变小,细胞核固缩、裂解,细胞膜皱褶、卷曲和出泡,并且有细胞膜包裹的凋亡小体生成。扫描电镜下观察细胞表面超微结构,发现细胞出现典型的凋亡形态学特征,细胞表面微绒毛大量减少,有的甚至消失,细胞体积变小,细胞膜皱缩,表面凸起,形成了大量的小泡,有的成为凋亡小体。

关键词: 苦荞麦, 蛋白质, 乳腺癌细胞, 凋亡

Abstract:

The inhibition effect of a protein fraction TBWSP31 prepared from tartary buckwheat as described in our previous study on the proliferation of Bcap37 cells was investigated by MTT assay, HE staining and scanning electron microscopic (SEM) observation. TBWSP31 exhibited a notable anti-tumor activity in dose- and time-dependent manners and was also sensitive to human mammary cancer cell Bcap37 with IC50 values of 43.37 (48 h)μg/mL and 19.75μg/mL (72 h), respectively. After treated with TBWSP31 for 48 h, Bcap37 cells revealed typical apoptotic characteristics. The HE-stained cells exhibited visible morphological changes such as nuclear fragmentation, chromatin condensation, plasma-membrane blebbing and production of apoptotic bodies under a light microscope. The SEM observations revealed that the decrease of villus, even partial disappearance, plasma-membrane blebbing and production of apoptotic bodies.

Key words: tartary buckwheat, protein, breast cancer cells, apoptotic

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