食品科学 ›› 2010, Vol. 31 ›› Issue (19): 325-328.doi: 10.7506/spkx1002-6630-201019071

• 营养卫生 • 上一篇    下一篇

小麦胚活性肽对D-半乳糖衰老模型小鼠抗氧化作用研究

陈 英1,朱科学1,彭 伟1,刘洪涛2,顾耀兴3,周惠明1 ,*   

  1. 1.江南大学食品学院 2.弥诺食品有限公司 3.江苏淮安新丰面粉有限公司
  • 收稿日期:2010-03-01 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 周惠明1 E-mail:hmzhou@jiangnan.edu.cn
  • 基金资助:

    江苏省科技支撑计划项目(BE 2009361-1)

Antioxidant Effect of Wheat Germ Bioactive Peptides in D-galactose-induced Aging Mice

CHEN Ying1,ZHU Ke-xue1,PENG Wei1,LIU Hong-tao2,GU Yao-xing3,ZHOU Hui-ming1,*   

  1. 1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    2. Minuo Food Co. Ltd., Yongcheng 476600, China ;3. Huai’an Xinfeng Flour Co. Ltd., Huai’an 223001, China
  • Received:2010-03-01 Online:2010-10-15 Published:2010-12-29
  • Contact: ZHOU Hui-ming1 E-mail:hmzhou@jiangnan.edu.cn

摘要:

目的:研究不同剂量小麦胚活性肽对D- 半乳糖衰老模型小鼠抗氧化作用。方法:将50 只昆明小鼠随机分为5 组(正常对照组,模型对照组,低、中、高剂量多肽组),除正常对照组外,其余4 组腹腔注射600mg/(kgbw·d) D- 半乳糖建立衰老模型,多肽组灌胃低、中、高剂量(200、800、1000mg/(kg bw·d)小麦胚活性肽, 实验周期45d,眼球取血处死小鼠,测定血清、脑、肝脏和心脏中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)和丙二醛(MDA)含量。结果:小麦胚活性肽能显著提高血清中T-AOC、GSH-Px、SOD 活力(P < 0.01)以及心脏中SOD 活力 和 T-AOC(P < 0.01);也能显著提高脑和肝脏中GSH-Px的活力(P < 0.05)和脑中T-AOC(P < 0.05);同时使血清和各组织中的MDA 含量显著降低(P < 0.05)。结论:说明小麦胚活性肽有较强的体内抗氧化活性。

关键词: 小麦胚活性肽, D- 半乳糖, 小鼠, 抗氧化, 体内

Abstract:

Objective: To explore the antioxidant effect of wheat germ bioactive peptides (WGBP) in aging mice induced by D-galactose. Methods: Fifty male Kunming mice were randomly divided into five groups, control group, model group, and WGBP low-, medium- and high-dose groups. Except the control group, all the other groups were subjected to subcutaneous injection of D-galactose solution at a dose of 600 mg/(kg bw·d). Mice were treated with WGBP at doses of 200, 800 mg/(kg bw·d) and 1000 mg/(kg bw·d) once a day for the creation of the WGBP low-, medium- and highdose groups. The activities of SOD and GSH-Px, T-AOC and the content of MDA in serum, brain, liver and heart were determined after a trial period of 45 days. Results: The activities of GSH-Px, SOD and T-AOC exhibited a significant increase (P < 0.01) in serum, and the activities of SOD and T-AOC also exhibited a significant increase in heart (P < 0.01) due to WGBP treatment. The activity of GSH-Px in brain and liver exhibited an obvious increase (P < 0.05); meanwhile, an obvious increase of SOD activity in brain was also observed (P < 0.05). Furthermore, the content of MDA in serum and the above organs revealed a reducing trend (P < 0.05). Conclusion: WGBP has good antioxidant effect in vivo as expected.

Key words: wheat germ bioactive peptides, D-galactose, mice, antioxidant effect, in vivo

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