食品科学 ›› 2010, Vol. 31 ›› Issue (19): 347-350.doi: 10.7506/spkx1002-6630-201019076

• 营养卫生 • 上一篇    下一篇

虾蛋白过敏实验动物模型的建立

王丽娟1 ,2,胡志和1 ,2,陈照丽2,周 汛2,吴海明2   

  1. 1.天津市食品生物技术重点实验室 2.天津商业大学生物技术与食品科学学院
  • 收稿日期:2010-06-22 修回日期:2010-08-30 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 王丽娟 E-mail:wljuan@tjcu.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划重大项目(2008BAD94B09)

Establishment of an Animal Model for Shrimp Protein Sensitivity Test

WANG Li-juan1,2,HU Zhi-he1,2,CHEN Zhao-li2,ZHOU Xun2,WU Hai-ming2   

  1. 1. Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China ;
    2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
  • Received:2010-06-22 Revised:2010-08-30 Online:2010-10-15 Published:2010-12-29
  • Contact: WANG Li-juan E-mail:wljuan@tjcu.edu.cn

摘要:

以卵白蛋白为阳性对照,以平衡盐溶液(PBS)为阴性对照,观察虾蛋白的全身过敏反应。同时,观察虾蛋白对致敏豚鼠离体回肠平滑肌过敏性收缩反应(Schultz-Dale 反应)的影响,以建立虾蛋白过敏实验动物模型。结果显示,用0.01~2g/100mL 卵白蛋白致敏并激发后,100% 豚鼠呈全身过敏反应阳性,0.1g/100mL 卵白蛋白组的豚鼠100% 死亡。相似质量浓度虾蛋白(0.01~1g/100mL)的全身过敏反应与卵白蛋白相近(与卵白蛋白组比较,P > 0.05);Schultz-Dale 反应的实验结果与全身过敏反应结果相似。由此,初步建立虾蛋白过敏实验的动物模型。

关键词: 虾蛋白, 卵白蛋白, 动物模型, 全身过敏反应, Schultz-Dale 反应

Abstract:

Egg albumin was used as the positive control and balanced salt solution (PBS) was used as the negative control to observe general allergic reactions of shrimp protein. Guinea pig was used to establish an animal model for shrimp protein sensitivity test by observing the effect of shrimp protein on isolated ileal smooth muscle contraction reaction (Schultz-Dale reaction). Results indicated that the general allergic reactions in each guinea pig were positive after stimulated by egg albumin at a concentration varying from 0.01 to 1 g/100mL. All the guinea pigs died after the stimulation with egg albumin at a concentration of 0.1 g/100mL. The general allergic reactions of shrimp protein were similar with those of egg albumin at the similar concentration range from 0.01 to 1 g/100mL. Meanwhile, the Schultz-Dale reaction results were also similar with those of systemic allergic reactions. Therefore, an animal model for shrimp protein sensitivity test has been successfully established.

Key words: shrimp protein, egg albumin, animal model, systemic allergic reaction, Schultz-Dale reaction

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