食品科学 ›› 2010, Vol. 31 ›› Issue (18): 14-17.doi: 10.7506/spkx1002-6630-201018004

• 工艺技术 • 上一篇    下一篇

梅花鹿鹿筋胶原蛋白提取工艺条件优化

张 鹤,赵 雨* ,徐云凤,李芸彤,赵大庆   

  1. 长春中医药大学中医药与生物工程研发中心
  • 收稿日期:2009-12-24 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 赵雨 E-mail:cnzhaoyu@yahoo.com.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2007BAI38B06);吉林省科技支撑计划项目(20096042)

Optimization of Extraction Conditions for Deer Tendon Collagen

ZHANG He,ZHAO Yu*,XU Yun-feng,LI Yun-tong,ZHAO Da-qing   

  1. Chinese Medicine and Bio-engineering Research Center, Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2009-12-24 Online:2010-09-25 Published:2010-12-29
  • Contact: ZHAO Yu*, E-mail:cnzhaoyu@yahoo.com.cn

摘要:

为应用胃蛋白酶酶解法从鹿筋中提取制备胶原蛋白。采用单因素试验和正交试验筛选出鹿筋胶原蛋白提取的最佳条件为醋酸体积分数5.5%、料液比1:7(mL/g)、浸提时间14h,胃蛋白酶与底物比1:1000(g/g)、酶解时间24h。验证实验结果表明,采用本方法提取的鹿筋胶原蛋白收率为74.56%,蛋白含量为65.54%。本实验优选所得梅花鹿鹿筋胶原蛋白提取工艺,方法简便,提取率高。

关键词: 鹿筋, 胶原蛋白, 胃蛋白酶, 高效凝胶色谱

Abstract:

Pepsin hydrolysis was used to extract and prepare collagen from deer tendons. The optimal extraction conditions were explored by single factor experiments and orthogonal experiments to be acetic acid concentration of 5.5%, material-to-liquid ratio of 1:9, extraction time of 10 h, pepsin-substrate ratio of 1:1000, and hydrolysis time of 24 h. The yield of deer tendon collagen was 74.56% and protein content was 65.54%. The optimized extraction method for deer tendon collagen was characteristic of simple operation and high extraction rate.

Key words: deer tendon, collagen, pepsin, HPGFC

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