食品科学 ›› 2010, Vol. 31 ›› Issue (18): 22-25.doi: 10.7506/spkx1002-6630-201018006

• 工艺技术 • 上一篇    下一篇

不同沉淀方法分离甘薯β-淀粉酶的研究

贾彦杰1,梁新红2,朱文学1,孙俊良2 ,* ,郭祖锋2   

  1. 1.河南科技大学食品与生物工程学院
    2.河南科技学院食品学院
  • 收稿日期:2010-05-21 修回日期:2010-07-20 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 孙俊良 E-mail:sjl@hist.edu.cn
  • 基金资助:

    河南省科技计划项目(102102210192);河南科技学院博士基金项目(09001)

Separation of β-amylase from Sweet Potato by Different Precipitation Methods

JIA Yan-jie1,LIANG Xin-hong2,ZHU Wen-xue1,SUN Jun-liang2,*,GUO Zu-feng2   

  1. 1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China;
    2. College of Food, Henan Institute of Science and Technology, Xinxiang 453003, China
  • Received:2010-05-21 Revised:2010-07-20 Online:2010-09-25 Published:2010-12-29
  • Contact: SUN Jun-liang E-mail:sjl@hist.edu.cn

摘要:

研究盐析法、有机溶剂沉淀法分离甘薯β- 淀粉酶的工艺条件,分析盐析饱和度、有机溶剂体积比和溶液pH 值对回收率及提纯倍数的影响。结果表明:硫酸铵饱和度在70% 时,酶活性回收率达到78.1%,提纯1.56倍;乙醇体积比4:1 时,酶活性回收率达到76.8%,提纯2.61 倍;当溶液pH4.5~5.0、丙酮体积比3:1 时,酶活性回收率可达到90.66%,提纯了3.29 倍,此条件下β- 淀粉酶分离效果最佳。

关键词: 甘薯, β-淀粉酶, 沉淀, 分离, 盐析

Abstract:

Salting out and organic solvent precipitation methods were solely used to precipitate β-amylase from the supernatant of sweet potato homogenate. The effects of saturation degree for salting out, volume ratio for organic solvents and pH on the recovery rate and purity of β-amylase were investigated. Results indicated that the specific activity ofβ-amylase reached up to 78.1% and its purity exhibited a 1.56-fold enhancement when the saturation degree of ammonium sulfate was 70%; the specific activity of β-amylase reached up to 76.8% and its purity exhibited a 2.61-fold enhancement when 4-fold volume of ethanol was added to the supernatant of sweet potato homogenate; and the highest separation efficiency was achieved and the specific activity ofβ-amylase reached up to 90.66% with a 3.29-fold enhancement in purity when pH varied from 4.5 to 5.0 and 3-fold volume of acetone was used instead of 4-fold volume of ethanol.

Key words: sweet potato, β-amylase, precipitation, separation, salting out

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