食品科学 ›› 2010, Vol. 31 ›› Issue (18): 26-30.doi: 10.7506/spkx1002-6630-201018007

• 工艺技术 • 上一篇    下一篇

响应曲面法优化醇提荷叶复方物超临界萃取残渣中黄酮的工艺

甘露菁,邓泽元*   

  1. 南昌大学 食品科学与技术国家重点实验室,高等研究院
  • 收稿日期:2009-12-18 修回日期:2010-06-25 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 甘露菁 E-mail:closer081@hotmail.com
  • 基金资助:

    江西省自然科学基金项目(2006GY0066);江西省教育厅科研课题(20030058)

Optimization of Ethanol Extraction of Total Flavonoids from the Leftover Residue of a Chinese Herbal Medicine Combination after Supercritical Carbon Dioxide Extraction Using Response Surface Methodology

GAN Lu-jing,DENG Ze-yuan   

  1. Institute for Advanced Study, State Key Laboratory of Food Science and Technology, Nanchang University,
    Nanchang 330047, China
  • Received:2009-12-18 Revised:2010-06-25 Online:2010-09-25 Published:2010-12-29
  • Contact: DENG Ze-yuan E-mail:closer081@hotmail.com

摘要:

对荷叶复方物超临界萃取后的残渣中黄酮进行醇提,用Box-Behnken 响应曲面设计法,以料液比、乙醇体积分数、温度及时间为自变量,以总黄酮得率为响应指标,用回归方程的方差分析检验模型的准确性。修正后最优化的条件为残渣10g 时,料液比1:20(g/mL) 、乙醇体积分数50%、温度80℃、时间80min。在此条件下总黄酮得率为15.97mg/g,提取3 次结果与理论预测值相近。因此,基于Box-Behnken 响应曲面法所得的优化提取工艺参数准确可靠,具有实用价值,可用于荷叶复方降脂产品的开发。

关键词: 响应曲面法, 黄酮, 残渣

Abstract:

Ethanol extraction was employed to extract total flavonoids from the leftover residue of a Chinese herbal medicine combination consisting of lotus leaves, cassia seeds, hawthorn fruit and oriental water plantain rhizome after supercritical carbon dioxide extraction. The optimal conditions for the extraction of total flavonoids were explored by response surface methodology. Box-Behnken design was applied to provide experimental data for establishing a mathematical regression model describing the relationship between the extraction rate of total flavonoids and four independent variables including material-to-liquid ratio, ethanol concentration, extraction temperature and extraction time. Correlation analysis of the established model was performed, and the results indicated that it could be employed to optimize the ethanol extraction of total flavonoids from the start material. Material-to-liquid ratio of 1:20, ethanol concentration of 50%, extraction temperature of 80 ℃ and extraction time of 80 min were found optimum. The experimental values of the extraction rate of total flavonoids under these optimum conditions were in good agreement with the predicted ones.

Key words: response surface methodology, flavonoids, residue

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