食品科学 ›› 2010, Vol. 31 ›› Issue (18): 41-45.doi: 10.7506/spkx1002-6630-201018010

• 工艺技术 • 上一篇    下一篇

中性蛋白酶酶解制备魔芋飞粉淀粉及其性质的研究

谭博文1,徐怀德1 ,* ,米林峰2   

  1. 1.西北农林科技大学食品科学与工程学院
    2.榆林市产品质量监督检验所
  • 收稿日期:2009-12-24 修回日期:2010-05-19 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 徐怀德 E-mail:xuhuaide@yahoo.com.cn
  • 基金资助:

    陕西省陕南突破发展基金项目(2006ZKC(二)04-34)

Preparation of Konjac Fly Powder by Neutral Protease Hydrolysis and Its Properties

TAN Bo-wen1,XU Huai-de1,*,MI Lin-feng2   

  1. 1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China;
    2. Yulin Products Quality Supervision and Inspection Institute, Yulin 719000, China
  • Received:2009-12-24 Revised:2010-05-19 Online:2010-09-25 Published:2010-12-29
  • Contact: Xu Huai-De E-mail:xuhuaide@yahoo.com.cn

摘要:

对中性蛋白酶酶解魔芋飞粉制备淀粉的方法及其性质进行研究。应用单因素和中心组合试验对酶解条件进行优化,用扫描电镜观察淀粉颗粒形貌,然后对淀粉糊的主要性质及指标进行测定。结果表明:中性蛋白酶酶解魔芋飞粉的最佳工艺参数为底物的质量分数2.5%、酶用量818.3U/g 底物、温度44.1℃、pH7.1、水解120min。制得淀粉纯度90.09%,其中直连淀粉含量18.20%,支链淀粉含量81.80%,淀粉糊具有较好的溶解度和透明度。魔芋淀粉颗粒为蚕豆形,颗粒粒径为1~9μm,平均为5μm,魔芋淀粉颗粒小不易发生凝沉现象。

关键词: 中性蛋白酶, 魔芋飞粉淀粉, 制备, 性质

Abstract:

Konjac fly powder was prepared by neutral protease hydrolysis from konjac starch and its properties were investigated. Results indicated that the optimal hydrolysis conditions were substrate concentration of 2.5%, alkaline protease concentration of 818.3 U/g, hydrolysis temperature of 44.1 ℃ and hydrolysis time of 120 min. The purity of prepared starch reached up to 90%, which contained 18.20% amylose and 81.80% amylopectin. Konjac starch paste exhibited excellent solubility and transparency. Konjac starch granules were broad bean shape with particle sizes ranging from 1 to 9μm, averaged at 5μm.

Key words: neutral protease, konjac fly powder starch, preparation, property

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