食品科学 ›› 2010, Vol. 31 ›› Issue (18): 95-98.doi: 10.7506/spkx1002-6630-201018022

• 工艺技术 • 上一篇    下一篇

氯霉素分子印迹聚合物微球的制备及色谱性能评价

史西志1,杨 华2,孙爱丽1,李德祥1,陈 炯1   

  1. 1.宁波大学生命科学与生物工程学院
    2.浙江万里学院生物与环境学院
  • 收稿日期:2010-05-19 修回日期:2010-08-24 出版日期:2010-09-25 发布日期:2010-12-29
  • 基金资助:

    宁波市自然科学基金项目(2009A610146);浙江省教育厅高校科研计划项目(Y200803423);
    国家“863”计划项目(2006AA10Z438)

Preparation and Chromatographic Evaluation of Molecularly Imprinted Polymer Microspheres for Chloramphenicol

SHI Xi-zhi1,YANG Hua2,SUN Ai-li1,LI De-xiang1,CHEN Jiong1   

  1. 1. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China;
    2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
  • Received:2010-05-19 Revised:2010-08-24 Online:2010-09-25 Published:2010-12-29

摘要:

以氯霉素(CAP)为模板分子、甲基丙烯酸N,N- 二乙基氨基乙酯(DEAEM)为功能单体、二甲基丙烯酸乙二醇酯(EGDMA)为交联剂、聚苯乙烯微球作为种球,采用两步溶胀聚合的方法制备得到了粒径均一的分子印迹聚合物微球(MIPMs)。结果表明:扫描电镜观察显示其表面为多孔结构;通过孔容和孔径研究表明,与非印迹聚合物微球(NIPMs)相比,MIPMs 具有更大的孔径和孔体积;进一步通过液相色谱对其选择性能进行评价,可知氯霉素、甲砜霉素、氟甲砜霉素在MeCN/Water/H3PO4(30:70:0.1,V/V)流动相条件下,3 种物质达到了较好的分离效果,获得的MIPMs 对3 种物质具有较好的选择性能。

关键词: 氯霉素, 分子印迹微球, 甲砜霉素, 氟甲砜霉素

Abstract:

A uniformly sized molecularly imprinted polymer microspheres (MIPMs) for chloramphenicol (CAP) has been prepared by a two-step swelling and polymerization method using 2-(Diethylamino) ethyl methacrylate (DEAEM), ethylene glycol dimethacrylate (EGDMA) and polystyrene microspheres as the functional monomer, cross-linker and seed particles, respectively. The porous surface of MIPMs could be clearly observed under scanning electron microscope (SEM). Furthermore, the MIPMs had a larger pore volume and pore diameter measured using BELSORP-miniII than the non-imprinted polymer microspheres (NIPMs) did. The selectivity of MIPMs was assessed by chromatographic method, and CAP, thiamphenicol (TAP) and florfenicol (FF) could reach a stronger separation on the MIPMs with a MeCN/water/H3PO4 mixture (30:70:0.1,V/V) as the mobile phase.

Key words: chloramphenicol, molecularly imprinted polymer microspheres, thiamphenicol, florfenicol

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