食品科学 ›› 2010, Vol. 31 ›› Issue (18): 108-111.doi: 10.7506/spkx1002-6630-201018025

• 工艺技术 • 上一篇    下一篇

响应面分析法优化海金沙多糖的提取工艺

武 芸,郑小江* ,卜贵军,曾 智   

  1. 湖北民族学院 生物资源保护与利用湖北省重点实验室
  • 收稿日期:2010-06-18 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 武芸 E-mail:wuyun2058sohu.com
  • 基金资助:

    国家民委科研项目(08HB04);湖北省科技厅创新团队项目(2009CDA155);2010 年恩施州科技指导项目

Extraction Optimization of Water-soluble Polysaccharides from the Shoot of Lygodium japonicum Using Response Surface Methodology

WU Yun,*,BU Gui-jun,ZENG Zhi   

  1. 郑小江
  • Received:2010-06-18 Online:2010-09-25 Published:2010-12-29
  • Contact: ZHENG Xiao-jiang E-mail:wuyun2058sohu.com

摘要:

利用响应面分析法对海金沙草多糖的提取工艺进行优化。在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理采用三因素三水平的响应面分析法,确定各工艺条件的影响因素,以海金沙多糖提取率为响应面和等高线,在分析各个因素的显著性和交互作用后,得出海金沙多糖浸提的最佳工艺条件为温度92℃、料液比1:25、浸提时间2.1h。在最佳提取条件下,提取2 次,海金沙草的多糖得率为16.475%。

关键词: 海金沙, 多糖, 提取, 响应面分析法

Abstract:

Based on single factor studies, a three-variable, three-level Box-Benhnken experimental design and response surface methodology were employed to optimize the extraction process of water-soluble polysaccharides from the shoot of Lygodium japonicum. Response surface and contour plots with polysaccharide yield as a function were obtained, and process conditions were analyzed for their significance for polysaccharide extraction and significance analysis of their interactions was also done. Extraction temperature of 92 ℃ and solid/liquid ratio of 1:25 for twice extraction for 2.1 h each time were found optimal. Under such conditions, a polysaccharide yield of 16.475% was obtained.

Key words: Lygodium japonicum, polysaccharide, extraction, response surface methodology

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