食品科学 ›› 2010, Vol. 31 ›› Issue (18): 224-226.doi: 10.7506/spkx1002-6630-201018051

• 分析检测 • 上一篇    下一篇

高纯度磷脂酰胆碱的制备及其脂肪酸分析

楼乔明,徐 杰,孙兆敏,薛长湖*   

  1. 中国海洋大学食品科学与工程学院
  • 收稿日期:2009-12-22 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 楼乔明 E-mail:louqm2005@163.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2008BAD94B04)

Preparation and Fatty Acid Analysis of Phosphatidylcholine with High Purity

LOU Qiao-ming,XU Jie,SUN Zhao-min,XUE Chang-hu*   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Received:2009-12-22 Online:2010-09-25 Published:2010-12-29
  • Contact: LOU Qiao-ming E-mail:louqm2005@163.com

摘要:

利用不同溶剂对大豆浓缩磷脂中各成分溶解性的差异,建立一种溶剂法制备高纯度大豆磷脂酰胆碱的新工艺。大豆浓缩磷脂通过丙酮脱油、乙醇提取、活性炭脱色、乙腈萃取,可制备纯度高达95% 以上的大豆磷脂酰胆碱。大豆磷脂酰胆碱的脂肪酸主要为多不饱和脂肪酸,其中亚油酸占总脂肪酸的70.71%、亚麻酸11.04%。

关键词: 溶剂法, 磷脂酰胆碱, 脂肪酸

Abstract:

Due to the difference in solubility of concentrated soybean phospholipids in different solvents, a solvent method to prepare phosphatidylcholine with high purity was established. Concentrated soybean phospholipids were subjected to oilremoval by acetone, isolation by ethanol, decoloration by active carbon, and extraction by acetonitrile. The purity of prepared phosphatidylcholine was up to 95%. Fatty acid compositions of soybean phosphatidylcholine were mainly composed of polyunsaturated fatty acids, and the contents of linoleic acid and linolenic acid were 70.71% and 11.04%, respectively.

Key words: solvent method, phosphatidylcholine, fatty acid

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