食品科学 ›› 2010, Vol. 31 ›› Issue (16): 16-20.doi: 10.7506/spkx1002-6630-201016004

• 工艺技术 • 上一篇    下一篇

大孔树脂纯化大果沙棘果渣总黄酮的工艺研究

焦 岩1,2,王振宇1,3,*   

  1. 1.东北林业大学林学院
    2.齐齐哈尔大学食品与生物工程学院 3.哈尔滨工业大学食品科学与工程学院
  • 收稿日期:2009-11-24 出版日期:2010-08-25 发布日期:2010-12-29
  • 通讯作者: 王振宇 E-mail:jiaoyan_3000@126.com
  • 基金资助:

    黑龙江省科技攻关项目(GB06B404-1)

Macroporous Adsorption Resin for the Purification of Flavonoids from Seabuckthorn Pomace

JIAO Yan1,2,WANG Zhen-yu1,3,*   

  1. 1.School of Forestry, Northeast Forestry University, Harbin 150040, China ;
    2.College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China;
    3.College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
  • Received:2009-11-24 Online:2010-08-25 Published:2010-12-29
  • Contact: WANG Zhen-yu E-mail:jiaoyan_3000@126.com

摘要:

目的:研究大孔树脂纯化大果沙棘果渣总黄酮的纯化工艺。方法:对7 种大孔吸附树脂纯化大果沙棘果渣总黄酮的效果进行比较,考察X-5 大孔树脂分离纯化大果沙棘果渣总黄酮的最佳工艺条件。结果:X-5 树脂纯化大果沙棘果渣总黄酮效果最佳,其最适工艺条件为:大果沙棘提取液上样质量浓度2mg/mL,吸附时间2h,用4BV 蒸馏水洗脱除去杂质,然后用4BV 70% 乙醇洗脱,树脂可重复利用5 次以上,此条件下纯化后总黄酮回收率最高为86.78%,纯度可由原来的11.6% 提高到34.1%。

关键词: 大孔树脂, 纯化, 大果沙棘果渣, 总黄酮

Abstract:

Considering overall adsorption and desorption capacity, X-5 resin was selected out of 7 types of macroporous resins to purify the crude flavonoids extract from seabuckthorn pomace. The optimum process conditions for purifying the crude flavonoids extract using X-5 resin were determined as follows: sample concentration 2 mg/mL, adsorption duration 2 h, amount of distilled water used for washing the resin 4 BV, and amount of 70% ethanol as desorption solvent 4 BV. Optimized purification gave a maximum recovery of flavonoids of 86.78% and an increase in the purity from 11.6% to 34.1%. X-5 resin was used repeatedly 6 times for flavonoids purification, and both the recovery and purity of flavonoids remained higher.

Key words: macroporous adsorption resin, purification, seabuckthorn pomace, flavonoids

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