食品科学 ›› 2010, Vol. 31 ›› Issue (16): 156-160.doi: 10.7506/spkx1002-6630-201016032

• 分析检测 • 上一篇    下一篇

顶空固相微萃取-气质联用方法分析龙眼中的挥发性化合物

张 义1,2,高 蓓1,2,徐玉娟1,温 婧1,潘思轶2,张名位2,3,*   

  1. 1.广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室
    2.华中农业大学食品科学技术学院
    3.广东省农业科学院农业生物技术研究所
  • 收稿日期:2009-03-02 修回日期:2010-06-11 出版日期:2010-08-25 发布日期:2010-12-29
  • 通讯作者: 张名位 E-mail:mwzhh@163.net
  • 基金资助:

    国家自然科学基金管理委员会- 广东省联合基金项目(u0731005);广东省自然科学基金团队项目(06200586);
    广东省教育部产学研结合项目(2008A090400015)

Determination of Volatile Compounds in ‘Chuliang’ Longan Using Headspace Solid-phase Microextraction and GC-MS

ZHANG Yi1,2,GAO Bei1,2,XU Yu-juan1,WEN Jing1,PAN Si-yi2,ZHANG Ming-wei2,3,*   

  1. 1. Research Institute of Sericulture and Farm Produce Processing, Guangdong Academy of Agricultural Sciences, Guangdong Open
    Access Laboratory of Agricultural Product Processing 2. College of Food Science and Technology,
    Huazhong Agricultural University3. Research Institute of Agricultural Biotechnology, Guangdong Academy of Agricultural Sciences
  • Received:2009-03-02 Revised:2010-06-11 Online:2010-08-25 Published:2010-12-29
  • Contact: ZHANG Ming-wei E-mail:mwzhh@163.net

摘要:

研究龙眼中挥发性化合物的检测方法。以龙眼(储良)为试材,采用顶空固相微萃取法提取挥发性成分,用气相色谱- 质谱联用仪进行检测,采用质谱和保留指数检索相结合的方法进行定性分析,加入内标环己酮进行半定量分析,对萃取样品温度、萃取纤维头吸附时间及脱附时间进行优化。结果表明:在对方法精密度的考察中,主要化合物峰面积相对标准偏差(RSD)为2.12%~9.08%;龙眼(储良)中共计检测出41 种挥发性化合物,主要成分为反-罗勒烯(81.2%)、乙醇(1.92%)、别- 罗勒烯(1.39%)、γ- 松油烯(0.30%)、β- 月桂烯(0.30%)、里那醇(0.26%)。顶空固相微萃取- 气质联用方法操作简单,能够很好地应用于龙眼芳香成分的检测。

关键词: 龙眼(储良), 顶空固相微萃取, 气相色谱- 质谱联用, 芳香物质

Abstract:

This paper describes a rapid headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometric (GC-MS) method for determining the volatile compounds in ‘Chuliang’ longan. Qualification was performed by both mass spectral and retention index library searching. For semi-quantification, an internal standard solution (IS) of cyclohexanone was used. Operating conditions for headspace solid-phase micro-extraction and GC-MS were optimized. The precision RSDs for the predominant compounds in ‘Chuliang’ longan ranged from 2.12% to 9.08%. Forty-one volatile compounds were detected in ‘Chuliang’ longan and trans-ocimene (81.2%), ethanol (1.92%), allo-ocimene (1.39%), γ-terpinene (0.30%), β-myrcene (0.30%) and linalool (0.26%) were the predominant compounds. The presented method is of simple operation and most suitable for the determination of volatile compounds in ‘Chuliang’ longan.

Key words: ‘Chuliang&rsquo, longan, headspace solid-phase microextraction, GC-MS, volatile compounds

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