食品科学 ›› 2010, Vol. 31 ›› Issue (16): 296-299.doi: 10.7506/spkx1002-6630-201016065

• 技术应用 • 上一篇    下一篇

固定化乳酸菌发酵胡萝卜酸奶工艺研究

吴 定,孙嘉文,姚明兰,路桂红   

  1. 南京财经大学食品科学与工程学院
  • 收稿日期:2010-04-13 出版日期:2010-08-25 发布日期:2010-12-29
  • 通讯作者: 吴定 E-mail:foodfermentation@163.com
  • 基金资助:

    江苏省教育厅自然科学基金项目(JDX20080481)

Optimization of Carrot Yoghurt Production by Fermentation of Immobilized Lactobacillus delbrueckii subsp bulgaricus and Streptococcus salivarius subsp thermophilus

WU Ding,SUN Jia-wen,YAO Ming-lan,LU Gui-hong   

  1. College of Food Science and Engineering, Nanjing University of Finance and Economices, Nanjing 210003, China
  • Received:2010-04-13 Online:2010-08-25 Published:2010-12-29
  • Contact: WU Ding E-mail:foodfermentation@163.com

摘要:

通过固定化乳酸菌发酵胡萝卜酸奶,建立新的胡萝卜酸奶发酵工艺。经过单因素试验和正交试验,确定固定化乳酸菌发酵胡萝卜酸奶的配方和发酵工艺。胡萝卜酸奶的配方:奶粉9g、胡萝卜13g、白砂糖8g、蒸馏水100mL。胡萝卜酸奶发酵工艺:固定化德氏乳杆菌保加利亚亚种与固定化唾液链球菌嗜热亚种的比为2:1 接种发酵液(菌种的总添加量为6g/100mL)、在37℃发酵培养7h、然后4℃冷藏12h。结果表明,采用固定化乳酸菌新技术可以发酵生产营养丰富、口感良好的胡萝卜酸奶。

关键词: 固定化唾液链球菌嗜热亚种, 固定化德氏乳杆菌保加利亚亚种, 胡萝卜酸奶配方, 发酵工艺

Abstract:

For better development of the carrot industry, carrot rich in various nutriments was used as fermentation substrate to product yoghurt by the fermentation of a mixed starter composed of immobilized Streptococcus salivarius subsp thermophilus and Lactobacillus delbrueckii subsp bulgaricus. The present study focused on developing a novel optimal process for carrot yoghurt production. Based on one-factor-at-a-time investigations, orthogonal array designs were employed to optimize fermentation medium composition and fermentation conditions. The optimal fermentation medium composition was found to be consist of milk powder 9 g, carrot 13 g, white sugar 8 g and distilled water 100 mL, and the optimal mixing ratio for immobilized Streptococcus salivarius subsp thermophilus and Lactobacillus delbrueckii subsp bulgaricus was 2:1 for the fermentation at 37 ℃ for 7 h using an inoculum size of 6% followed by cold storage at 4 ℃ for 12 h. The product obtained under the above optimal conditions was rich in various nutrients and had a pleasant mouth feel.

Key words: immobilized Streptococcus salivarius subsp thermophilus, immobilized Lactobacillus delbrueckii subsp bulgaricus, carrot yoghurt formula, fermentation technology

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