食品科学 ›› 2010, Vol. 31 ›› Issue (14): 10-13.doi: 10.7506/spkx1002-6630-201014003

• 工艺技术 • 上一篇    下一篇

响应面法优化提取甜橙皮渣中果胶的研究

马亚琴1,2,3,孙志高1,2,*,吴厚玖1,2,周志钦1,3   

  1. 1.西南大学柑桔研究所
    2.国家柑桔工程技术研究中心 3.西南大学园艺园林学院
  • 收稿日期:2009-11-05 修回日期:2010-04-06 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 孙志高 E-mail:cpro@163.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2007BAD47B06);柑桔学重庆市市级重点实验室开放基金项目(CKLC200805)

Extraction Optimization of Pectin from Sweet Orange Peels Using Response Surface Methodology

MA Ya-qin1,2,3,SUN Zhi-gao1,2,*,WU Hou-jiu1,2,ZHOU Zhi-qin1,3   

  1. 1. Citrus Research Institute, Southwest University, Chongqing 400712, China ;
    2. National Citrus Engineering Research Center, Chongqing 400712, China;
    3. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400712, China
  • Received:2009-11-05 Revised:2010-04-06 Online:2010-07-15 Published:2010-12-29
  • Contact: SUN Zhi-gao1, E-mail:cpro@163.com

摘要:

通过单因素试验研究料液比、提取温度、提取时间和pH 值4 因素对甜橙皮渣中果胶得率的影响。在单因素试验的基础上,采用三因素三水平的Box-Behnken Design(BBD)中心试验设计研究响应值以及最佳变量的组合。结果表明:随着pH 值的降低和温度的增加果胶得率增加;处理时间和料液比对果胶得率也有积极的影响。通过响应面法综合评价提取甜橙皮渣中果胶的最佳提取条件为温度90℃、时间1.3h、pH1.1,在此条件下的果胶得率预测值为0.998%。

关键词: 甜橙皮渣, 果胶, 提取, 响应面

Abstract:

To obtain the optimal process conditions for the extraction of pectin from sweet orange peels, the effects of materialto- liquid ratio, extraction temperature, length of extraction and pH on pectin yield were investigated by single factor method and based on this, a 3-factor, 3-level Box-Behnken experimental design coupled with response surface methodology was employed to optimize extraction temperature, length of extraction and pH. The results showed that pectin yield increased with decreasing pH and increasing temperature and length of extraction and material-to-liquid ratio had positive correlation with pectin yield. The optimum values of extraction temperature, length of extraction time and pH were determined to be 90 ℃, 1.3 h and 1.1, respectively.

Key words: sweet orange peels, pectin, extraction, response surface methodology

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