食品科学 ›› 2010, Vol. 31 ›› Issue (14): 22-25.doi: 10.7506/spkx1002-6630-201014006

• 工艺技术 • 上一篇    下一篇

孵育法富集麦胚多肽工艺参数优化

杨润强1,龚小峰1,顾振新1 ,* ,吴志坚2   

  1. 1.南京农业大学食品科技学院 2.常州五星禾绿蔬菜食品有限公司
  • 收稿日期:2010-02-22 修回日期:2010-05-21 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 顾振新1 E-mail:guzx@njau.edu.cn
  • 基金资助:

    江苏省科技支撑计划项目(BE2009303)

Optimization of Incubation Conditions for Polypeptide-enriched Wheat Germ

YANG Run-qiang1,GONG Xiao-feng1,GU Zhen-xin1,*,WU Zhi-jian2   

  1. 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Changzhou Wuxxin Helv Shucai Shipin Co. Ltd., Changzhou 213016, China
  • Received:2010-02-22 Revised:2010-05-21 Online:2010-07-15 Published:2010-12-29
  • Contact: GU Zhen-xin1,*, E-mail:guzx@njau.edu.cn

摘要:

对孵育法富集麦胚多肽工艺进行优化。结果表明:孵育时间、温度、孵育液pH 值及液料比对麦胚孵育液中多肽含量有显著影响。采用Design Expert 中Box-Behnken 试验设计对上述因素进行响应面优化,结果表明当孵育时间6h、孵育温度49℃、孵育液pH3 及液料比10:1(mL/g)时为最佳组合,麦胚孵育液中多肽含量高达88.46mg/g,是原料麦胚的2.98 倍。

关键词: 麦胚, 孵育, 肽含量, 蛋白酶活力

Abstract:

In this study, incubation conditions including incubation time, incubation temperature, initial pH and liquid-tomaterial ratio (mL/g) for the polypeptide accumulation in wheat germ were investigated. The single factor investigations indicated that all of these conditions had significant effect on the polypeptide content of wheat germ. The response surface optimization based on a 4-factor, 3-level Box-Behnken experimental design revealed that the optimal values of incubation time and temperature, initial pH and liquid-to-material ratio were determined to be 6 h, 49 ℃, 3.0 and 10:1, respectively. After the incubation under such conditions, the maximum peptide polypeptide content of wheat germ reached up to 88.46 mg/g, 2.98 times higher than before incubation.

Key words: wheat germ, incubation, polypeptide content, protease activity

中图分类号: