食品科学 ›› 2010, Vol. 31 ›› Issue (14): 32-36.doi: 10.7506/spkx1002-6630-201014008

• 工艺技术 • 上一篇    下一篇

响应曲面法优化苹果渣多酚纯化工艺

白雪莲1,岳田利1,*,章华伟2,陈 岩1   

  1. 1.西北农林科技大学食品科学与工程学院
    2.浙江工业大学药学院
  • 收稿日期:2009-11-24 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 岳田利1 E-mail:ytl6503@163.com
  • 基金资助:

    陕西省攻关项目(2008K08-04);陕西省科技创新工程重大专项(2008ZDKG-04)

Optimization of NKA-Ⅱ Macroporous Resin Purification of Polyphenols from Apple Pomace Using Response Surface Methodology

BAI Xue-lian1,YUE Tian-li1,*,ZHANG Hua-wei2,CHEN Yan1   

  1. 1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China;
    2. School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China
  • Received:2009-11-24 Online:2010-07-15 Published:2010-12-29
  • Contact: YUE Tian-li1 E-mail:ytl6503@163.com

摘要:

以苹果渣来源的苹果多酚为原料,采用响应曲面法(RSM)研究NKA-Ⅱ树脂对苹果多酚的纯化工艺及吸附操作中吸附速率、上样液pH 值、上样液质量浓度及其交互作用对苹果多酚吸附效果的影响,解析操作中解吸速率、乙醇体积分数、洗脱剂pH 值及其交互作用对苹果多酚解吸效果的影响。利用SAS8.0 软件处理数据,确定了NKA-Ⅱ树脂纯化苹果渣多酚最优吸附条件为吸附速率1.065mL/min、上样液pH3.19、上样液质量浓度1.421mg/mL;最佳解吸条件为解吸速率0.91mL/min、乙醇体积分数73.99%、洗脱剂pH8.90,在最佳纯化条件下苹果多酚纯度是38.45%。

关键词: 苹果渣, 苹果多酚, 响应曲面, 大孔树脂

Abstract:

In order to optimize the NKA-Ⅱ macroporous resin purification of polyphenols from apple pomace, Box-Behnken experimental design coupled with multiple regression analysis was employed to establish separate mathematical models stimulating the adsorption and desorption behaviors of NKA-Ⅱ resin and based on the established models, response surface methodology (RSM) was employed to analyze the respective effects of interactions among adsorption conditions and among desorption conditions on the adsorption and desorption of apple polyphenols onto NKA-Ⅱ resin. The optimum dynamic adsorption conditions were as follows: sample flow rate 1.065 mL/min, pH 3.19, sample concentration 1.421 mg/mL, and the optimum dynamic desorption conditions were as follows: desorption flow rate 0.91 mL/min, ethanol concentration 73.99% and pH 8.90. The optimized purification provided a purity of apple polyphenols of 38.45%.

Key words: apple pomace, apple polyphenols, RSM, macroporous resin

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