食品科学 ›› 2010, Vol. 31 ›› Issue (14): 42-46.doi: 10.7506/spkx1002-6630-201014010

• 工艺技术 • 上一篇    下一篇

胃蛋白酶水解酪蛋白制备ACE抑制肽的条件

张 艳,胡志和*,闫 星,樊 华   

  1. 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室
  • 收稿日期:2009-12-18 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 胡志和 E-mail:hzhihe@tjcu.edu.cn
  • 基金资助:

    天津市科技支撑计划重点项目(07ZCKFNC00200)

Pepsin Hydrolysis of Casein for the Preparation of ACE Inhibitory Peptides

ZHANG Yan,HU Zhi-he*,YAN Xing,FAN Hua   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotecnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Received:2009-12-18 Online:2010-07-15 Published:2010-12-29
  • Contact: HU Zhi-he E-mail:hzhihe@tjcu.edu.cn

摘要:

研究胃蛋白酶水解酪蛋白制备ACE 抑制肽的工艺条件。采用胃蛋白酶水解酪蛋白制备ACE 抑制肽,检测其水解产物的ACE 抑制率,以ACE 抑制率为指标,通过正交试验设计法,确定最终水解条件为水解温度37℃、pH3.0、酶与底物质量比1:100、底物质量分数7%,水解产物ACE 抑制率为92.5%,其IC50 值为0.24mg/mL。酪蛋白的水解度与ACE 抑制率之间并非简单的相关性。

关键词: 酪蛋白, ACE抑制活性, 胃蛋白酶

Abstract:

The optimal pepsin hydrolysis of casein for preparing ACE inhibitory peptides was explored by single factor and orthogonal array design methods. The optimal hydrolysis parameters were determined to be: 37 ℃, pH 3.0, enzyme-tosubstrate ratio 1:100, and substrate concentration 7%. The optimized pepsin hydrolysis yielded a product presenting 92.5% ACE inhibition ratio and 0.24 mg/mL IC50. The correlation between hydrolysis degree of casein and ACE inhibition ratio of its hydrolysates was complicated.

Key words: casein, ACE inhibitory activity, pepsin

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