食品科学 ›› 2010, Vol. 31 ›› Issue (14): 101-105.doi: 10.7506/spkx1002-6630-201014022

• 工艺技术 • 上一篇    下一篇

响应面分析法优化莲花蜂花粉多糖提取工艺研究

刘 洁1,2,缪晓青1,2,3,*   

  1. 1.福建农林大学蜂学学院 2.福建农林大学蜂疗研究所 3.福建省神蜂科技开发有限公司
  • 收稿日期:2009-02-24 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 缪晓青 E-mail:bingjie825@163.com

Optimization of Polysaccharides Extraction from Lotus Bee Pollen by Response Surface Methodology

LIU Jie1,2,MIAO Xiao-qing1,2,3,*   

  1. 1. College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China ;2. Institute of Apitherapy, Fujian Agriculture and Forestry University, Fuzhou 350002, China ;3. Fujian Shenfeng Science and Technology Co. Ltd., Fuzhou 350002, China
  • Received:2009-02-24 Online:2010-07-15 Published:2010-12-29
  • Contact: MIAO Xiao-qing E-mail:bingjie825@163.com

摘要:

为研究水提法提取莲花蜂花粉多糖的条件,在单因素试验的基础上,根据Box-Behnken 试验设计原理,选取料液比、浸提时间和浸提温度三因素三水平进行响应面分析,建立多糖提取率的二次回归方程,得到提取工艺的优化组合条件。结果表明:料液比、浸提时间和浸提温度对莲花蜂花粉多糖提取率都有显著影响,当提取工艺条件为料液比1:9.4(g/mL)、浸提时间2.4h、浸提温度81.6℃、浸提2 次时,莲花蜂花粉多糖提取率预测值为1.2201%、验证值为1.2317%。

关键词: 莲花蜂花粉多糖, 响应面分析, 工艺优化

Abstract:

This paper reports the use of single factor method and Box-Behnken experimental design combined with response surface methodology for optimizing the water extraction yield of lotus bee pollen polysaccharides (LBPP). In the optimization, a quadratic regression model predicting LBPP yield as a function of 3 extraction conditions, i.e., solid-to-liquid ratio, length of extraction and extraction temperature was set up and the effects of these 3 extraction conditions and their pairwise interactions on LBPP yield were evaluated. All of these extraction conditions had significant effect on LBPP yield and their optimum values were determined as follows: solid-to-liquid ratio 1:9.4 (g/mL) and extraction temperature 81.6 ℃ for an extraction duration of 2.4 h repeated twice. Under such conditions, the predicted and experimental values of LBPP yield were 1.2201% and 1. 2317%, respectively.

Key words: lotus bee pollen polysaccharides, response surface analysis, optimization

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