食品科学 ›› 2010, Vol. 31 ›› Issue (14): 106-110.doi: 10.7506/spkx1002-6630-201014023

• 工艺技术 • 上一篇    下一篇

枇杷果浆酶解工艺的响应面法优化

张 瑶1,蒲 彪1,*,刘 云2,刘兴艳1   

  1. 1.四川农业大学食品学院
    2.西南林学院资源学院
  • 收稿日期:2009-10-21 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 蒲彪 E-mail:pubiao2002@yahoo.com.cn

Using Response Surface Methodology for Optimizing Pectinase Hydrolysis of Loquat Slurry

ZHANG Yao1,PU Biao1,*,LIU Yun2,LIU Xing-yan1   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China ;
    2. Faculty of Natural Resources, Southwest Forestry College, Kunming 650224, China
  • Received:2009-10-21 Online:2010-07-15 Published:2010-12-29
  • Contact: PU Biao E-mail:pubiao2002@yahoo.com.cn

摘要:

以五星枇杷为原料,在单因素试验的基础上,确定果胶酶质量浓度、酶解温度和酶解时间3 个因素的取值范围,并根据中心组合设计原理和响应面分析枇杷果浆酶解工艺最优条件。结果表明:果胶酶用量、酶解温度和酶解时间对出汁率和透光率有较显著的影响;在果胶酶用量166.05mg/kg、酶解温度40.60℃、酶解时间5.64h的最优条件下,枇杷果浆的实测出汁率为91.17%、透光率为76.24%,回归模型的预测值与实测值的相对误差小于1%,此回归方程与实际情况拟合较好。

关键词: 枇杷果浆, 酶解工艺, 响应面法, 模型预测

Abstract:

The goal of this study was to develop an optimum process for hydrolyzing loquat slurry. A series of single factor experiments were carried out to address the effects of enzyme concentration, hydrolysis temperature and length of hydrolysis on juice yield and transmittance. Central composite design coupled with response surface methodology was employed to optimize these 3 hydrolysis conditions. It was indicated that all of the conditions significantly influenced juice yield and transmittance and that their optimum values were determined as follows: enzyme concentration 166.05 mg/kg, hydrolysis temperature 40.60 ℃ and hydrolysis duration 5.64 h. Under these optimum conditions, the experimental values of juice yield and transmittance were 91.17% and 76.24%, respectively, presenting a relative error of less than 1% when compared with the predicted counterparts.

Key words: loquat slurry, enzyme technique, response surface methodology (RSM), predicted pattern

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