食品科学 ›› 2010, Vol. 31 ›› Issue (14): 158-160.doi: 10.7506/spkx1002-6630-201014035

• 分析检测 • 上一篇    下一篇

乳酸脱氢酶测定试剂盒鉴定复原乳

赵 越,庞广昌* ,朱健春   

  1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院
  • 收稿日期:2009-11-02 修回日期:2010-04-26 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 庞广昌 E-mail:pgc@tjcu.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD04A00)

Identification of Reconstituted Milk Using a Commercially Available Lactate Dehydrogenase Kit

ZHAO Yue,PANG Guang-chang*,ZHU Jian-chun   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of
    Commerce, Tianjin 300134, China
  • Received:2009-11-02 Revised:2010-04-26 Online:2010-07-15 Published:2010-12-29
  • Contact: PANG Guang-chang E-mail:pgc@tjcu.edu.cn

摘要:

利用乳酸脱氢酶测定试剂盒测定巴氏杀菌乳、超高温灭菌乳和复原乳中乳酸脱氢酶活性的差异以鉴定复原乳。结果显示:巴氏杀菌乳中乳酸脱氢酶的活性显著高于超高温灭菌乳和复原乳。该方法操作简单、快速准确, 值得推广。

关键词: 乳酸脱氢酶, 巴氏杀菌乳, 超高温灭菌乳, 复原乳

Abstract:

Lactate dehydrogenase exists widely in human and animal tissues and body fluids. Due to the temperature influence in different processing technologies, the activity of lactate dehydrogenase was different in pasteurized milk, ultra-high temperature- treated milk and reconstituted milk. A commercially available lactate dehydrogenase kit was used to determine the activity of lactate dehydrogenase in pasteurized milk, ultra-high temperature-treated milk and reconstituted milk for identifying reconstituted milk. Results indicated that the activity of lactate dehydrogenase of pasteurized milk was significantly higher than those of ultra-high temperature-treated milk and reconstituted milk. This method is of easy operation, rapidity and high accuracy and consequently, deserves to be popularized.

Key words: lactate dehydrogenase, pasteurized milk, ultra-high temperature treated milk, reconstituted milk

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