食品科学 ›› 2010, Vol. 31 ›› Issue (14): 178-181.doi: 10.7506/spkx1002-6630-201014040

• 分析检测 • 上一篇    下一篇

沙苑子提取物中多酚含量的测定

张清安1 , 2,范学辉3,张志琪2,马学燕1,陈 瑜1,王 军1   

  1. 1.陕西师范大学食品工程与营养科学学院
    2.陕西师范大学化学与材料科学学院
    3.陕西师范大学体育学院
  • 收稿日期:2009-10-14 修回日期:2010-03-18 出版日期:2010-07-15 发布日期:2010-12-29
  • 通讯作者: 张清安 E-mail:qinganzhang@snnu.edu.cn
  • 基金资助:

    教育部博士点基金项目(20050718011)

Determination of Polyphenols in Different Organic Solvent Extracts of Semen Astragali complanati

ZHANG Qing-an1,2,FAN Xue-hui3,ZHANG Zhi-qi2,MA Xue-yan1,CHEN Yu1,WANG Jun1   

  1. 1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China ;
    2. School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an 710062, China ;
    3. College of Sport, Shaanxi Normal University, Xi’an 710062, China
  • Received:2009-10-14 Revised:2010-03-18 Online:2010-07-15 Published:2010-12-29
  • Contact: ZHANG Qing-an1 E-mail:qinganzhang@snnu.edu.cn

摘要:

研究Folin-Ciocalteu 比色法测定沙苑子中多酚含量的优化条件,并以优化的条件测定沙苑子不同溶剂提取物中多酚的含量。结果表明:Folin-Ciocalteu 显色剂(经稀释10 倍)用量1.00mL、7.5g/100mL Na2CO3 用量3.00mL、反应温度25℃、反应时间120min、测定波长647nm 为沙苑子中多酚含量测定的最佳条件,所用没食子酸标准品在10~100μg/mL 范围内与吸光度呈良好的线性关系(y=0.0081x - 0.0249,R2=0.9996);沙苑子甲醇、乙醇和丙酮溶液提取物的质量浓度为1g/100mL 左右时较适合测定其多酚的含量。

关键词: 沙苑子, 提取物, 多酚, 测定

Abstract:

In order to provide an optimal Folin-Ciocalteu assay for determining polyphenols in different organic solvent (ethanol aqueous solution, acetone aqueous solution and methanol aqueous solution all at 60% cocentration) extracts of Semen Astragali complanati, some Folin-Ciocalteu assay conditions were optimized. The results indicated that the optimal conditions for the determination of polyphenols by Folin-Ciocalteu were as follows: 10-fold diluted Folin-Ciocalteu reagent amount 1.00 mL, 7.5 g/100 mL Na2CO3 amount 3 mL, reaction temperature 25 ℃, reaction time 120 min, and determination wavelength 647 nm. A good linear relationship was observed between absorbance and gallic acid concentration within the range of 10-100 μg/mL (y = 0.0081x -0.0249, R2 = 0.9996). In addition, accurate contents of polyphenols in methanol extract, ethanol extract and acetone extract of Semen Astragali complanati diluted to a concentration of 1 g/100 mL was determined by this method.

Key words: Semen Astragali complanati, extract, polyphenols, determination

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