食品科学 ›› 2010, Vol. 31 ›› Issue (13): 88-92.doi: 10.7506/spkx1002-6630-201013021

• 基础研究 • 上一篇    下一篇

黄鲫(Setipinna taty)蛋白酶解液的抑菌活性及稳定性研究

宋 茹1,2,韦荣编2,3,谢 超2,汪东风1,*   

  1. 1.中国海洋大学食品科学与工程学院
    2.浙江海洋学院食品与药学学院
    3.中国药科大学生命科学与技术学院
  • 收稿日期:2009-11-16 修回日期:2010-03-19 出版日期:2010-07-01 发布日期:2010-12-29
  • 通讯作者: 汪东风 E-mail:wangdf@ouc.edu.cn;wangdf@mail.ouc.edu.cn
  • 基金资助:

    舟山市科技计划项目(Y20082086)

Antibacterial Activity and Stability of Half-Fin Anchovy (Setipinna taty) Protein Hydrolysate

SONG Ru1,2 WEI Rong-bian2,3 XIE Chao2 WANG Dong-feng1,*   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
    2. School of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China
    3. School of Life Sciences and Technology, China Pharmaceutical University, Nanjing 210018, China
  • Received:2009-11-16 Revised:2010-03-19 Online:2010-07-01 Published:2010-12-29
  • Contact: WANG Dong-feng E-mail:wangdf@ouc.edu.cn;wangdf@mail.ouc.edu.cn

摘要:

以黄鲫为原料测定其氨基酸组成,比较风味蛋白酶、胰蛋白酶、胃蛋白酶、碱性蛋白酶和木瓜蛋白酶水解黄鲫蛋白所得酶解液对大肠杆菌的抑菌作用,并对抑菌效果最强的蛋白酶酶解液进行抑菌稳定性研究。结果表明:黄鲫蛋白必需氨基酸含量丰富,其中胃蛋白酶的水解液对大肠杆菌抑菌作用强,相对分子质量主要分布在3000~1000;黄鲫胃蛋白酶酶解液对热稳定,酸性低pH值可增强其抑菌效果,可耐受胰蛋白酶和β-内酰胺酶处理,该酶解液有作为天然抗菌剂应用的前景。

关键词: 黄鲫, 蛋白酶解液, 抑菌活性, 稳定性

Abstract:

Half-fin anchovy (Setipinna taty) was determine for its amino acid composition. The antibacterial activities of half-fin anchovy hydrolysates separately derived from flavor protease, trypsin, pepsin, alkaline protease and papain digestion were evaluated using Escherichia coli. Half-fin anchovy was rich in essential amino acids. The pepsin hydrolysate exhibited the highest antibacterial activity against Escherichia coli and its relative molecular mass ranged from 3000 to 1000. In addition, pepsin hydrolyzed half-fin anchovy also exhibited high thermal stability and enhanced antibacterial activity at lower acidic pH values. Moreover, pepsin hydrolyzed half-fin anchovy exhibited high tolerance to trypsin andβ-lactamase. Therefore, pepsin hydrolyzed half-fin anchovy appears to be a promising natural antibacterial agent.

Key words: half-fin anchovy, protein hydrolysate, antibacterial activity, stability

中图分类号: