关键词: 马兰提取物, 黄酮类化合物, 抗氧化性, 清除自由基

Abstract:

The present study was carried out to investigate the antioxidant activity and free radical scavenging activities of water and ethanol extracts from Kalimeris indica (L.) Sch-Bip. DPPH, hydroxyl and superoxide anion free radical as well as hydrogen peroxide scavenging assays were carried out to evaluate the antioxidant potential of both of the extracts. Along with this, their reducing powers and ferrous iron chelating capacities were determined. Flavonoid compounds were the major compounds in both of the extracts. The order for the free radical scavenging activities and ferrous iron chelating capacity of the water extract from Kalimeris indica (L.) Sch-Bip was superoxide anion free radicals (IC50 2.2 μg/mL), H2O2 (IC50 2.8μg/mL), hydroxyl free radicals (IC50 5.6μg/mL), DPPH (IC50, 8.5μg/mL) and ferrous iron chelating capacity (IC50, 28.0μg/mL). As for the ethanol extract, its free radical scavenging activities and ferrous iron chelating capacity were ranked in the following order: H2O2 (IC50, 2.0μg/mL), superoxide anion fee radicals (IC50, 2.1μg/mL), hydroxyl free radicals (IC50, 2.5μg/mL), DPPH free radicals (IC50, 5.6μg/mL) and metal chelating activity (IC50, 51.2μg/mL). Considering the above results collectively, both of the extracts from Kalimeris indica (L.) Sch-Bip appear to be a promising antioxidant.

Key words: Kalimeris indica (L.) Sch-Bip, flavonoids, antioxidant activity, free radical scavenging capacity