食品科学 ›› 2010, Vol. 31 ›› Issue (13): 228-232.doi: 10.7506/spkx1002-6630-201013052

• 生物工程 • 上一篇    下一篇

链霉菌G-HD-4 产黑色素发酵条件的优化

郑晨娜1,罗婉妹1,陈慧勤1,吴晓岚1,方柏山2   

  1. 1.泉州医学高等专科学校基础医学部 2. 厦门大学化学化工学院
  • 收稿日期:2009-10-14 修回日期:2010-04-13 出版日期:2010-07-01 发布日期:2010-12-29
  • 通讯作者: 郑晨娜 E-mail:nanaz_0@126.com

Optimal Fermentation Conditions for Melanin Production by Streptomyces G-HD-4

ZHENG Chen-na1,LUO Wan-mei1,CHEN Hui-qin1,WU Xiao-lan1,FANG Bai-shan2   

  1. 1. Department of Basic Medical Science, Quanzhou Medical College, Quanzhou 362000, China ;
    2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361021, China
  • Received:2009-10-14 Revised:2010-04-13 Online:2010-07-01 Published:2010-12-29
  • Contact: ZHENG Chen-na1 E-mail:nanaz_0@126.com

摘要:

为提高链霉菌G-HD-4 的黑色素产生量,以L- 酪氨酸为底物,对该菌种产黑色素的工艺条件进行研究,通过单因素试验和均匀设计试验,得到最佳产黑色素的发酵条件为:马铃薯15g/100mL、乳糖2.5g/100mL、干酪素2.07g/100mL、MgSO4 0.11g/100mL、KH2PO4 0.25g/100mL、L- 酪氨酸 0.25g/100mL、以接种量为6%(体积分数),培养基起始pH 值为6.0,装液量为25mL,28℃,150r/min 振荡培养5d,黑色素产量可达(13.4 ± 0.05)g/L,比在基础培养基中的黑色素产量(5.78g/L)提高约2.3 倍。

关键词: 链霉菌G-HD-4, 黑色素, 发酵, 均匀设计

Abstract:

In order to improve melanin production by Streptomyces G-HD-4 fermentation, the optimal conditions for fermenting L-tyrosine by this strain were investigate by single factor and uniform design methods. The optimal medium compositions (on the basis of 100 mL medium) were 15 g potato, 2.5 g lactose, 2.07 g casein, 0.25 g KH2PO4, 0.11 g MgSO4, and 0.25 g L-tyrosine. The optimal fermentation process was that 25 mL of medium was contained in a 250 mL flask for 5-day fermentation at initial pH 6.0, 28 ℃ and 150 r/min shaking speed. As a result, the maximum melanin yield reached up to (13.4 ± 0.05) g/L, approximately 2.3 times higher than that obtained using preoptimization fermentation medium.

Key words: Streptomyces G-HD-4, melanin, fermentation, uniform-design

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