食品科学 ›› 2010, Vol. 31 ›› Issue (12): 6-10.doi: 10.7506/spkx1002-6630-201012002

• 工艺技术 • 上一篇    下一篇

响应曲面法优化亚临界水提取葡萄籽原花青素的工艺研究

李 超1,郑 义1,王卫东1,王乃馨1,杨日福2   

  1. 1.徐州工程学院食品工程学院 2.华南理工大学理学院
  • 收稿日期:2009-10-11 修回日期:2010-02-03 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 李超 E-mail:chaoge002@163.com
  • 基金资助:

    国家自然科学基金项目(20776047);徐州工程学院培育项目 (XKY2008322)

Optimization of Subcritical Water Extraction of Proanthocyanidins from Grape Seed by Response Surface Methodology

LI Chao1,ZHENG Yi1,WANG Wei-dong1,WANG Nai-xin1,YANG Ri-fu2   

  1. 1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China ;
    2. School of Science, South China University of Technology, Guangzhou 510640, China
  • Received:2009-10-11 Revised:2010-02-03 Online:2010-06-15 Published:2010-12-29
  • Contact: LI Chao1 E-mail:chaoge002@163.com

摘要:

采用自行设计的适合工业化应用的2L 亚临界水提取装置对葡萄籽中原花青素的亚临界水提取工艺进行优化,并与其他提取方法进行对比。结果表明:亚临界水提取原花青素的最佳工艺参数为提取温度151℃、提取时间21min 和提取压力12MPa,在此条件下,原花青素得率为3.88%;与传统索氏提取法和乙醇回流提取法相比,亚临界水提取法具有提取时间短、效率高等优点;所设计的2L 亚临界水提取装置自动化程度高、操作简便。

关键词: 亚临界水提取, 原花青素, 响应曲面法

Abstract:

In order to develop a new technology to extract proanthocyanidins, a 2 L equipment of subcritical water extraction (SWE) suitable for industrial production was designed and used to extract proanthocyanidins from grape seeds. SWE conditions for the extraction of proanthocyanidins were optimized by response surface methodology based on Box-Behnken experimental design, followed by a comparison between this technique and other extraction techniques. The results indicated that the optimal SWE conditions were determined as follows: extraction temperature 151 ℃ and extraction pressure 12 MPa for an extraction duration of 21 min; under such condtions, the extraction yield of proanthocyanidins was 3.88%. SWE required shorter length of extraction time and yielded a higher efficiency when compared with conventional Soxhlet extraction and ethanol reflux extraction. The designed 2 L equipment of SWE was highly automatic and convenient to operate.

Key words: response surface methodology, subcritical water extraction, proanthocyanidins

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