食品科学 ›› 2010, Vol. 31 ›› Issue (12): 49-52.doi: 10.7506/spkx1002-6630-201012011

• 工艺技术 • 上一篇    下一篇

响应曲面法优化桔梗皂甙提取工艺

徐丽萍,李笑梅,王 鑫   

  1. 哈尔滨商业大学食品工程学院
  • 收稿日期:2009-09-23 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 徐丽萍 E-mail:xuliping@163.com
  • 基金资助:

    黑龙江省自然科学基金项目(C200509);哈尔滨市科技局后备学科带头人基金项目(2005AFXXJ026)

Using Response Surface Analysis for Optimizing Ultrasonic-assisted Extraction of Saponins from the Roots of Platycodon grandiflorum

XU Li-ping,LI Xiao-mei,WANG Xin   

  1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
  • Received:2009-09-23 Online:2010-06-15 Published:2010-12-29
  • Contact: XU Li-ping, E-mail:xuliping@163.com

摘要:

以桔梗为原料,对超声辅助提取桔梗皂甙的工艺进行优化研究。利用 Box-Behnken 的中心组合设计原理及响应面法(RSM)分析建立二次回归模型,对料液比(桔梗粉末:水)、提取时间和提取温度三因素进行优化组合。确定超声辅助提取桔梗皂甙的最佳工艺条件为料液比1:9.75(g/mL)、超声温度35℃、超声时间31min。在该最佳工艺条件下,桔梗皂甙的提取率为4.64%,有效提高了桔梗皂甙的提取率。

关键词: 桔梗, 皂甙, 响应曲面, 提取

Abstract:

Saponins in the roots of Platycodon grandiflorum were extracted with distilled water under the assistance of ultrasonic, and the extraction process was optimized by using response surface analysis based on Box-Behnken design to examine the effects of extraction conditions including material-to-liquid ratio, length of ultrasonic treatment time and extraction temperature on saponin yield, which were reflected by a quadratic regression model. The results showed that the optimal values of the above extraction conditions were as follows: material-to-liquid ratio 1: 9.75 (g/mL), length of ultrasonic treatment time 31 min and extraction temperature 35 ℃. Under these conditions, the saponin yield was 4.64%, higher than before the optimization.

Key words: Platycodon grandiflorum, saponins, response surface analysis, extraction

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