食品科学 ›› 2010, Vol. 31 ›› Issue (12): 53-57.doi: 10.7506/spkx1002-6630-201012012

• 工艺技术 • 上一篇    下一篇

超声辅助提取花生红衣中多酚物质及其抗氧化活性的研究

赵 萍1,2,林樱姬2,金征宇1,王 雅2,王 莉2,亓文静2   

  1. 1.江南大学食品学院 2.兰州理工大学生命科学与工程学院
  • 收稿日期:2009-10-11 修回日期:2010-01-31 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 赵 萍 E-mail:pingzhaogdqg@lut.cn
  • 基金资助:

    甘肃省自然科学研究基金计划项目(0803RJZA044)

Ultrasonic-assisted Extraction and Antioxidant Evaluation of Polyphenols from Peanut Seed Testa

ZHAO Ping1,2,LIN Ying-ji2,JIN Zheng-yu1,WANG Ya2,WANG Li2,QI Wen-jing2   

  1. 1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
  • Received:2009-10-11 Revised:2010-01-31 Online:2010-06-15 Published:2010-12-29
  • Contact: ZHAO Ping1 E-mail:pingzhaogdqg@lut.cn

摘要:

目的:研究花生红衣中多酚类物质的提取工艺及抗氧化活性。方法:采用超声辅助提取方法,通过单因素和正交试验对花生红衣中多酚类物质(PTP)的提取工艺进行优化;采用还原力和Rancimat 法对其抗氧化活性进行研究。结果:PTP 最佳提取工艺为料液比1:16(g/mL)、乙醇溶液体积分数80%、提取时间10min、提取功率240W;提取因素影响大小顺序为料液比>乙醇溶液体积分数>提取时间>提取功率;在相同质量浓度条件下,纯化后的花生红衣多酚溶液PTP 的还原力强于VC 溶液的还原力;其对猪油抗氧化活性最优的质量浓度为150mg/L;相同质量浓度(150mg/L)条件下,其诱导时间长于VC、没食子酸溶液,而弱于BHT 溶液。

关键词: 花生红衣, 多酚, 超声辅助提取, 抗氧化性能

Abstract:

In this paper, it reports the use of one-factor-at-a-time and orthogonal array design methods to optimize the ultrasonicassisted extraction of polyphenols from peanut seed testa (PTP), followed by the evaluation of their antioxidant effects by reducing power assay and Rancimat assay. The results indicated that the optimum conditions for the extraction of polyphenols were determined to be: material-to-liquid ratio 1:16 (g/mL), ethanol concentration 80%, ultrasonic power 240 W and length of extraction time 10 min. The above conditions exhibited different effects on polyphenol extraction in the following order: material-to-liquid ratio > ethanol concentration > length of extraction time > ultrasonic power. Under the same concentrations, the reducing power of the purified PTP was superior to that of VC. The concentration of the purified PTP providing the best protective effect against lipid oxidation in lard was found to be 150 mg/L. Addition of the purified PTP at 150 mg/L resulted in longer induction time of oxidation of lard than that of VC or gallic acid, but was inferior to that of BHT. In conclusion, PTP appears to provide a promising natural antioxidant.

Key words: peanut testa (PT), polyphenols, ultrasonic-assisted extraction, antioxidant effect

中图分类号: