关键词: 反相液相色谱, 新疆野蔷薇, 黄酮, 芦丁, 槲皮素

Abstract:

A simple and rapid liquid chromatographic (LC) method was developed for determining total flavonoids in Rosa multiflora Thunb from Xinjiang. A mixture of methanol, isopropanol and deionized water was used for the chromatographic separation at a flow rate of 1 mL/min. The detection wavelength was set at 360 nm. Baseline separation was achieved for rutin and quercetin within 4 min. The contents of rutin and quercetin in Rosa multiflora Thunb from Xinjiang were 17.7 μg/g and 94.7μg/g (calculated on the basis of dry weight). Mean recoveries for rutin and quercetin at the spike level of 100μg/mL were 95.6% and 97.3%, respectively, and the RSDs were both below 5%. The repeatability of the developed method was good. As a conclusion, it is applicable for rapid determination of flavonoid compounds in Rosa multiflora Thunb, like rutin and quercetin.

Key words: flavonoids, liquid chromatography, quercetin, Rosa multiflora Thunb., rutin