食品科学 ›› 2010, Vol. 31 ›› Issue (12): 182-184.doi: 10.7506/spkx1002-6630-201012042

• 分析检测 • 上一篇    下一篇

GC-MS法分析蚕蛾油与蚕蛹油的脂肪酸组成

陈智毅,刘学铭,吴娱明,邹宇晓,施 英   

  1. 广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室
  • 收稿日期:2009-07-06 修回日期:2009-11-27 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 陈智毅 E-mail:fezychen@scut.edu.cn
  • 基金资助:

    国家现代农业产业技术体系建设专项;广东省科技攻关项目(2007A020200003-10)

GC-MS Analysis of Fatty Acid Composition of Silkworm Male Moths and Pupa

Chen Zhi-yi   

  1. Guangdong Open Access Laboratory of Agricultural Product Processing, Sericulture and Agri-Food Research Institute,
    Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
  • Received:2009-07-06 Revised:2009-11-27 Online:2010-06-15 Published:2010-12-29
  • Contact: Chen Zhi-yi E-mail:fezychen@scut.edu.cn

摘要:

采用超临界CO2 萃取雄蚕蛾与蚕蛹中的油脂,经甲酯化处理用气相色谱- 质谱联用仪进行分析,在雄蚕蛾油中分离鉴定出15 种脂肪酸,含量最高的是α- 亚麻酸(36.71%),其中直链饱和脂肪酸(SFA)54.52%;单不饱和脂肪酸(MUFA)6.89%;多不饱和脂肪酸(PUFA)38.63%。在蚕蛹油中共分离鉴定出14 种脂肪酸成分,含量最高的是亚油酸(39.03%),其次是α- 亚麻酸(33.22%),其中直链饱和脂肪酸(SFA)占25.73%;单不饱和脂肪酸(MUFA)占2.12%;多不饱和脂肪酸(PUFA)占72.25%。

关键词: 蚕蛾, 蚕蛹, 脂肪酸, 气相色谱-质谱法(GC-MS)

Abstract:

In this study, we used GC-MS to analyze the fatty acid composition of essential oils extracted from silkworm male moths and pupa with supercritical CO2 after methylation. Fifteen fatty acids were separated and identified in silkworm male moths oil, containing 54.52% of saturated fatty acids (SFA) and 6.89% of mono-unsaturated fatty acids (MUFA) and 38.63% of polyunsaturated fatty acids (PUFA), among whichs alpha linlenic acid presented the highest relative content (up to 36.71%). Fourteen fatty acids were identified in silkworm pupa oil, among which, the relative content of linoleic acid was the highest (up to 39.03%), followed by alpha linlenic acid (33.22%); SFA, MUFA and PUFA accounted for 25.73%, 2.12% and 72.25%.

Key words: silkworm moths, silkworm pupa, fatty acid, GC-MS

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