食品科学 ›› 2010, Vol. 31 ›› Issue (11): 11-14.doi: 10.7506/spkx1002-6630-201011003

• 基础研究 • 上一篇    下一篇

高压CO2酸化杀菌机理的研究

周先汉1,宋俊骅1,曾庆梅2,张 安1,程丽梅1,胡行军1,邹旭鹏1   

  1. 1.合肥工业大学生物与食品工程学院 2.合肥工业大学 农产品生物化工教育部工程研究中心
  • 收稿日期:2009-10-23 出版日期:2010-06-01 发布日期:2010-12-29
  • 通讯作者: 宋俊骅 E-mail:huasongjun@126.com
  • 基金资助:

    国家自然科学基金项目(30871739;30571304);安徽省教育厅重点科研项目(KJ2007A099)

Sterilization Mechanism of High-pressure Carbon Dioxide-caused Acidification

ZHOU Xian-han1 SONG Jun-hua2 ZENG Qing-mei1 ZHANG An1 CHENG Li-mei1 HU Xing-jun1 ZOU Xu-peng1   

  1. 1.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
    2.Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China
  • Received:2009-10-23 Online:2010-06-01 Published:2010-12-29
  • Contact: SONG Jun-hua E-mail:huasongjun@126.com

摘要:

以大肠杆菌及其低活性P-ATPase型和F-ATPase型大肠杆菌为研究对象,通过对比实验初步研究高压CO2杀菌过程的酸化机理。结果表明,高压CO2对突变型大肠杆菌的致死效果比对正常型大肠杆菌的致死效果都要好,H+-ATPase的活性对杀菌效果具有显著性影响,说明在高压CO2杀菌过程中,由于CO2气体渗透入细菌的细胞内,使胞内发生酸化,致使细菌死亡。

关键词: 高压CO2, 杀菌机理, P-ATPase, F-ATPase, 大肠杆菌

Abstract:

The sterilization mechanism of high-pressure carbon dioxide-caused acidification was studied using a wild-type strain of Escherichia coli and two mutant strains derived from it with low activity of P-ATPase or F-ATPase. The results showed that high-pressure carbon dioxide under the identical condition exhibited better lethal effect on both the mutant strains than on the wild-type strain. Meanwhile, a remarkable effect of H+-ATPase activity on sterilization was observed. Therefore, these investigations indicated that high-pressure carbon dioxide could result in intercellular acidification to cause bacterial death during sterilization.

Key words: high-pressure carbon dioxide, sterilization mechanism, P-ATPase, F-ATPase, Escherichia coli

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